In Vitro Packaging of Bacteriophage T7 DNA Requires ATP

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1982 Jul 1

PMID 7109030

Citations 4

Authors

W E Masker

Affiliations

Soon will be listed here.

Abstract

Removal of nucleoside triphosphates from extracts prepared from bacteriophage T7-infected Escherichia coli results in a stringent requirement for added ATP to form infective phage particles by in vitro packaging of bacteriophage T7 DNA. Optimal packaging efficiency was achieved at a concentration of about 1.25 mM. Other nucleoside triphosphates could be substituted for ATP, but none of the common nucleoside triphosphates was as effective as ATP in promoting in vitro encapsulation.

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