Human Monocyte Binding of Homologous Monomer and Complexed IgG

Overview

Journal Immunology

Specialty Allergy & Immunology

Date 1982 Aug 1

PMID 7106843

Citations 8

Authors

S D Carter

R G Leslie

W G Reeves

Affiliations

Soon will be listed here.

Abstract

Human peripheral blood monocyte binding of homologous IgG has been studied using monomer and covalently cross-linked dimer and trimer. A competitive radioassay using enriched monocytes was employed to determine the association constant (Ka) and the number of receptor sites per cell. Monomer and dimer bound with very similar Ka values (7.15 X 10(7) and 7.14 X 10(7)LM-1) whereas a slightly higher figure was obtained with trimer (8.9 X 10(7)LM-1). The number of receptor sites for dimer and trimer (90.5 and 107.5 X 10(3)/cell, respectively) were much greater than for monomer (14.5 X 10(3)/cell). Inhibition of dimer and trimer binding by monomer gave heterogenous Scatchard plots, each with two components. These findings suggest that human peripheral blood monocytes possess two types of Fc receptor, one of high affinity binding monomer and complexes and one of low affinity which predominantly binds complexes.

Citing Articles

Quantitative studies of Fc receptors on human monocytes: characterization by binding of homologous and heterologous monomeric IgG and soluble immune complexes of different composition.

Rasmussen J, Brandslund I, Leslie R, Svehag S Immunology. 1983; 49(3):537-44.

PMID: 6862526 PMC: 1454305.

Mononuclear phagocytes in rheumatoid arthritis: Fc-receptor expression by peripheral blood monocytes.

Carter S, Bourne J, Elson C, Hutton C, Czudek R, Dieppe P Ann Rheum Dis. 1984; 43(3):424-9.

PMID: 6742906 PMC: 1001364. DOI: 10.1136/ard.43.3.424.

The stimulation of superoxide anion production in guinea-pig peritoneal macrophages and neutrophils by phorbol myristate acetate, opsonized zymosan and IgG2-containing soluble immune complexes.

Baxter M, Leslie R, Reeves W Immunology. 1983; 48(4):657-65.

PMID: 6299935 PMC: 1454078.

Mechanism of inhibition of immunoglobulin G-mediated phagocytosis by monoclonal antibodies that recognize the Mac-1 antigen.

Brown E, Bohnsack J, Gresham H J Clin Invest. 1988; 81(2):365-75.

PMID: 2963020 PMC: 329578. DOI: 10.1172/JCI113328.

A receptor for monomeric IgG2b on rat macrophages.

Denham S, Barfoot R, Jackson E Immunology. 1987; 62(1):69-74.

PMID: 2958407 PMC: 1453711.

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