Nucleotide Sequence and Its Character of Cistron Coding for the 30 K Protein of Tobacco Mosaic Virus (OM Strain)

The nucleotide sequence of cloned cDNA copies of the common strain of tobacco mosaic virus RNA corresponding to the 2,000 nucleotides at the 3'-end was determined. The 30 K protein cistron was revealed to be located at residues 687-1,493 from the 3'-end. The 30 K protein is composed of 267 amino acids and is probably a basic protein. The 5' flanking regions of both the coat protein and the 30 K protein cistrons were very U-rich, and a homology was found between the sequence around the capping site of the coat protein mRNA and the sequence upstream from the 30 K protein cistron.