Effect of Electrical Stimulation of the Testis on the Nucleus Praeopticus Pars Magnocellularis of the Frog, Rana Tigrina

Overview

Journal Cell Tissue Res

Specialties Anatomy & Histology
Cell Biology

Date 1982 Jan 1

PMID 7094013

Authors

N Subhedar

V V Alandikar

Affiliations

Soon will be listed here.

Abstract

The changes in the nucleus praeopticus (NPO) pars magnocellularis of the frog Rana tigrina were studied after electrical stimulation of the left testis. After a 3-min stimulation, the neurones of the nucleus showed a statistically significant increase in the nuclear and cellular diameters. The lateral neurones in particular showed a complete loss of Nissl substance and other acute chromatolytic changes. In the aldehyde fuchsin stained preparations, however, the perikarya appeared filled with clumps of intensely stained neurosecretory material (NSM) and disintegrating cell nuclei. The NSM was discharged in the axons, resulting in an increase in number and size of the Herring bodies. These changes were abolished in frogs whose testes had been pre-anaesthetized with xylocaine. Based on these observations, the possible existence of an afferent pathway from the testis to the NPO pars magnocellularis is suggested.

References

Roberts W, Steinberg M, Means L . Hypothalamic mechanisms for sexual, aggressive, and other motivational behaviors in the opossium, Didelphis virginiana. J Comp Physiol Psychol. 1967; 64(1):1-15. DOI: 10.1037/h0024805. View

OZTAN N, Gorbman A . The hypophysis and hypothalamo-hypophyseal neurosecretory system of larval lampreys and their responses to light. J Morphol. 1960; 106:243-61. DOI: 10.1002/jmor.1051060302. View

RAO P . The effect of electrical stimulation of olfactory tract on the nucleus preopticus in the catfish Heteropneustes fossilis (Bloch). Experientia. 1970; 26(12):1377-8. DOI: 10.1007/BF02113043. View

Baumgarten H, HOLSTEIN A . [Adrenergic innervation in the testis and epididymis of the swam (Cygnus olor)]. Z Zellforsch Mikrosk Anat. 1968; 91(3):402-10. View

Brooks C, Ishikawa T, Koizumi K, Lu H . Activity of neurones in the paraventricular nucleus of the hypothalamus and its control. J Physiol. 1966; 182(1):217-31. PMC: 1357467. DOI: 10.1113/jphysiol.1966.sp007820. View

RAO P, Betole U, Subhedar N . Effect of electrical stimulation of the optic nerve on the nucleus preopticus and nucleus lateralis tuberis of the catfish, Clarias batrachus (Linn.). Neuroendocrinology. 1980; 30(4):232-7. DOI: 10.1159/000123006. View

Gresik E . Fine structural evidence for the presence of nerve terminals in the testis of the teleost, Oryzias latipes. Gen Comp Endocrinol. 1973; 21(1):210-3. DOI: 10.1016/0016-6480(73)90173-1. View

Ciriello J, CALARESU F . Monosynaptic pathway from cardiovascular neurons in the nucleus tractus solitarii to the paraventricular nucleus in the cat. Brain Res. 1980; 193(2):529-33. DOI: 10.1016/0006-8993(80)90182-1. View

Brooks C, Koizumi K, Zeballos G . A study of factors controlling activity of neurons within the paraventricular, supraoptic and ventromedian nuclei of the hypothalamus. Acta Physiol Lat Am. 1966; 16:83-96. View

10.

Peter R, Paulencu C . Involvement of the preoptic region in gonadotropin release-inhibition in goldfish, Carassius auratus. Neuroendocrinology. 1980; 31(2):133-41. DOI: 10.1159/000123064. View

11.

Vullings H, Kers J . The optic tracts of Rana temporaia and a possible retino-preoptic pathway. Z Zellforsch Mikrosk Anat. 1973; 139(2):179-200. DOI: 10.1007/BF00306521. View

12.

Unsicker K . Innervation of the testicular interstitial tissue in reptiles. Z Zellforsch Mikrosk Anat. 1973; 146(1):123-38. DOI: 10.1007/BF00306762. View

13.

Dierickx K, VANDESANDE F, Roggen D . THE REACTION OF DIFFERENT AREAS OF THE MAGNOCELLULAR PREOPTIC NUCLEUS OF RANA TEMPORARIA UPON PROGRESSIVE DEHYDRATION. Z Zellforsch Mikrosk Anat. 1964; 62:98-101. DOI: 10.1007/BF00339054. View

14.

Baumgarten H, Falck B, HOLSTEIN A, Owman C, Owman T . Adrenergic innervation of the human testis, epididymis, ductus deferens and prostate: a fluorescence microscopic and fluorimetric study. Z Zellforsch Mikrosk Anat. 1968; 90(1):81-95. DOI: 10.1007/BF00496704. View

15.

Gerendai I, Rotsztejn W, Marchetti B, Kordon C, Scapagnini U . Unilateral ovariectomy-induced luteinizing hormone-releasing hormone content changes in the two halves of the mediobasal hypothalamus. Neurosci Lett. 2009; 9(4):333-6. DOI: 10.1016/0304-3940(78)90204-5. View

16.

Stetson M . Hypothalamic regulation of gonadotropin release in female Japanese quail. Z Zellforsch Mikrosk Anat. 1972; 130(3):411-28. DOI: 10.1007/BF00306952. View

17.

Demski L, Knigge K . The telencephalon and hypothalamus of the bluegill (Lepomis macrochirus): evoked feeding, aggressive and reproductive behavior with representative frontal sections. J Comp Neurol. 1971; 143(1):1-16. DOI: 10.1002/cne.901430102. View

18.

Brooks C, Ushiyama J, LANGE G . Reactions of neurons in or near the supraoptic nuclei. Am J Physiol. 1962; 202:487-90. DOI: 10.1152/ajplegacy.1962.202.3.487. View

19.

Dierickx K . The total extirpation of the preoptic magno-cellular nucleus of Rana temporaria. Arch Int Pharmacodyn Ther. 1963; 143:268-75. View

20.

Jasinski A, Gorbman A, HARA T . Rate of movement and redistribution of stainable neurosecretory granules in hypothalamic neurons. Science. 1966; 154(3750):776-8. DOI: 10.1126/science.154.3750.776. View