Enhancement of Sister-chromatid Exchanges by Tumour Promoters

Overview

Journal Br J Cancer

Specialty Oncology

Date 1982 May 1

PMID 7082559

Citations 2

Authors

M Currens

P T Iype

Affiliations

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Abstract

The effect of the tumour promoters TPA, phenobarbitone and saccharin on the production of sister-chromatid exchanges (SCE) was studied. TPA produced a small but significant increase of SCE in Chinese hamster cell lines V-79 and CHO, and in a hybrid clone formed by fusion of CHO with rat liver epithelial cells. The enhancement of SCE by TPA was not affected by the type of culture medium, heat-inactivated bovine serum, or the batch of TPA. In the presence of exogenous L-cysteine the enhancement of SCE was reduced. TPA also increase the uptake of 2-deoxyglucose by the cells, to an extent similar to that of the SCE enhancement. This enhancement of SCE by TPA may be explained partly through the formation of free radicals, and partly through alterations in the cell-surface membrane and/or a transient delay in the cell-cycle progression. The other tumour promoters, phenobarbitone and saccharin, also enhanced both SCE and the uptake of 2-deoxyglucose in V-79 cells.

Citing Articles

Effects on mouse embryos of in utero exposure to saccharin: teratogenic and chromosome effects.

Dropkin R, Salo D, Tucci S, KAYE G Arch Toxicol. 1985; 56(4):283-7.

PMID: 3994513 DOI: 10.1007/BF00295169.

Enhancement of carcinogen-induced mutations or recombinations by 12-O-tetradecanoyl-phorbol-13-acetate in the mammalian spot test.

Fahrig R J Cancer Res Clin Oncol. 1987; 113(1):61-6.

PMID: 3818779 DOI: 10.1007/BF00389968.

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