Variation in the Sensitivity of Human Lymphocytes to DNA-damaging Agents Measured by Sister Chromatid Exchange Frequency

Overview

Journal Hum Genet

Specialty Genetics

Date 1982 Jan 1

PMID 7076244

Citations 2

Authors

P E Crossen

Affiliations

Soon will be listed here.

Abstract

Peripheral blood lymphocytes from normal donors were exposed to mitomycin C 0.01 microgram/ml, proflavine 0.4 microgram/ml, and 3H-uridine 3.7 Bq/ml and analyzed for the incidence of sister chromatid exchanges (SCEs). Cellular proliferation in the presence of these three agents was also measured by determining the number of first, second, and third divisions present. All donors showed a significant increase in SCE following treatment with mitomycin C, whereas only two-thirds showed an increase following treatment with proflavine and only half, with 3H-uridine. The variation in response was not related to either the age or sex of the donor. Cellular proliferation was likewise affected by all three agents. 3H-uridine caused the greatest reduction in the number of metaphases. There was no correlation between the sensitivity to one agent and another, nor was there any correlation the reduction in the number of metaphases and the increase in the number of exchanges.

Citing Articles

Characterization of the distribution of sister chromatid exchange frequencies: implications for research design.

Hirsch B, McGue M, cervenka J Hum Genet. 1984; 65(3):280-6.

PMID: 6698551 DOI: 10.1007/BF00286518.

Cytogenetic effects during extracorporeal photopheresis treatment of two patients with cutaneous T-cell lymphoma.

Peterseim U, Kuster W, Gebauer H, Meschig R, Plewig G Arch Dermatol Res. 1991; 283(2):81-5.

PMID: 2069416 DOI: 10.1007/BF00371613.

References

Honeycombe J . The effects of busulphan on the chromosomes of normal human lymphocytes. Mutat Res. 1978; 57(1):35-49. DOI: 10.1016/0027-5107(78)90232-4. View

Ikushima T, Wolff S . Sister chromatid exchanges induced by light flashes to 5-bromodeoxyuridine- and 5-iododeoxyuridine substituted Chinese hamster chromosomes. Exp Cell Res. 1974; 87(1):15-9. DOI: 10.1016/0014-4827(74)90521-7. View

Krepinsky A, Heddle J, German J . Sensitivity of Bloom's syndrome lymphocytes to ethyl methanesulfonate. Hum Genet. 1979; 50(2):151-6. DOI: 10.1007/BF00390236. View

Crossen P . The effect of acridine compounds on sister-chromatid exchange formation in cultured human lymphocytes. Mutat Res. 1979; 68(3):295-9. DOI: 10.1016/0165-1218(79)90161-7. View

LATT S, Stetten G, Juergens L, Buchanan G, GERALD P . Induction by alkylating agents of sister chromatid exchanges and chromatid breaks in Fanconi's anemia. Proc Natl Acad Sci U S A. 1975; 72(10):4066-70. PMC: 433139. DOI: 10.1073/pnas.72.10.4066. View

Setlow R . Repair deficient human disorders and cancer. Nature. 1978; 271(5647):713-7. DOI: 10.1038/271713a0. View

Crossen P, Morgan W . The effects of beta-radiation on sister-chromatid exchanges in cultured human lymphocytes. Mutat Res. 1979; 62(1):125-9. DOI: 10.1016/0027-5107(79)90225-2. View

Crossen P, Morgan W . Lymphocyte proliferation in Down's syndrome measured by sister chromatid differential staining. Hum Genet. 1980; 53(3):311-3. DOI: 10.1007/BF00287048. View

Schneider E, GILMAN B . Sister chromatid exchanges and aging. III. The effect of donor age on mutagen-induced sister chromatid exchange in human diploid fibroblasts. Hum Genet. 1979; 46(1):57-63. DOI: 10.1007/BF00278902. View

10.

Evans H . Some facts and fancies relating to chromosome structure in man. Adv Hum Genet. 1977; 8:347-438. DOI: 10.1007/978-1-4615-8267-0_5. View

11.

Wolff S, Rodin B, Cleaver J . Sister chromatid exchanges induced by mutagenic carcinogens in normal and xeroderma pigmentosum cells. Nature. 1977; 265(5592):347-9. DOI: 10.1038/265347a0. View

12.

Pero R, Bryngelsson C, Mitelman F, Kornfalt R, Thulin T, Norden A . Interindividual variation in the responses of cultured human lymphocytes to exposure from DNA damaging chemical agents: interindividual variation to carcinogen exposure. Mutat Res. 1978; 53(3):327-41. DOI: 10.1016/0165-1161(78)90005-5. View

13.

LATT S . Sister chromatid exchanges, indices of human chromosome damage and repair: detection by fluorescence and induction by mitomycin C. Proc Natl Acad Sci U S A. 1974; 71(8):3162-6. PMC: 388642. DOI: 10.1073/pnas.71.8.3162. View