Article: Serum concanavalin-A binding in rheumatoid arthritis

A nephelometric assay of concanavalin-A binding of serum acute phase proteins (con-A binding) has been used in cross-sectional and sequential studies of disease activity in rheumatoid arthritis (RA). Con-A binding correlated well with blood viscosity, C-reactive protein, and other individual acute phase reactants in patients with active RA. Twenty-four patients were treated for 6 months with D-penicillamine and assessed clinically and seriologically. Clinical improvement was accompanied by significant falls in both C-reactive protein and con-A binding, although the serological changes did not always occur in parallel in individual patients. The advantages of this simple, cheap assay of acute phase proteins are discussed.

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References

1.

LAURELL C . Quantitative estimation of proteins by electrophoresis in agarose gel containing antibodies. Anal Biochem. 1966; 15(1):45-52. DOI: 10.1016/0003-2697(66)90246-6. View

2.

So L, Goldstein I . Protein-carbohydrate interaction. IV. Application of the quantitative precipitin method to polysaccharide-concanavalin A interaction. J Biol Chem. 1967; 242(7):1617-22. View

3.

MCCONKEY B, Crockson R, Crockson A . The assessment of rheumatoid arthritis. A study based on measurements of the serum acute-phase reactants. Q J Med. 1972; 41(162):115-25. View

4.

MCCONKEY B, Crockson R, Crockson A, Wilkinson A . The effects of some anti-inflammatory drugs on the acute-phase proteins in rheumatoid arthritis. Q J Med. 1973; 42(168):785-91. View

5.

Amos R, Constable T, Crockson R, Crockson A, MCCONKEY B . Rheumatoid arthritis: relation of serum C-reactive protein and erythrocyte sedimentation rates to radiographic changes. Br Med J. 1977; 1(6055):195-7. PMC: 1604383. DOI: 10.1136/bmj.1.6055.195. View

6.

Uhlenbruck G, Baldo B, Steinhausen G, Schwick H, Chatterjee B, Horejsi V . Additional precipitation reactions of lectins with human serum glycoproteins. J Clin Chem Clin Biochem. 1978; 16(1):19-23. DOI: 10.1515/cclm.1978.16.1.19. View

7.

MCCONKEY B, Davies P, Crockson R, Crockson A, Butler M, Constable T . Effects of gold, dapsone, and prednisone on serum C-reactive protein and haptoglobin and the erythrocyte sedimentation rate in rheumatoid arthritis. Ann Rheum Dis. 1979; 38(2):141-4. PMC: 1000338. DOI: 10.1136/ard.38.2.141. View

8.

Warren C, Whicher J, Kohn J . The use of concanavalin A to measure acute phase proteins by laser nephelometry. J Immunol Methods. 1980; 32(2):141-50. DOI: 10.1016/0022-1759(80)90067-8. View

9.

MCCONKEY B, Amos R, Durham S, Forster P, Hubball S, Walsh L . Sulphasalazine in rheumatoid arthritis. Br Med J. 1980; 280(6212):442-4. PMC: 1600457. DOI: 10.1136/bmj.280.6212.442. View

10.

Nakamura S, Tanaka K, Murakawa S . Specific protein of legumes which reacts with animal proteins. Nature. 1960; 188:144-5. DOI: 10.1038/188144b0. View

11.

Harris H, Robson E . Precipitin reactions between extracts of seeds of Canavalia ensiformis (Jack Bean) and normal and pathological serum proteins. Vox Sang. 1963; 8:348-55. View

Serum Concanavalin-A Binding in Rheumatoid Arthritis