Alpha 2.0
Login Register
» Articles » PMID: 7045165

A Flexible Microprocessor System for the Measurement of Cell Size

Overview
Journal J Clin Pathol
Specialty Pathology
Date 1982 Jun 1
PMID 7045165
Citations 9
Authors
J M Round
D A Jones
R H EDWARDS
Affiliations
Soon will be listed here.
Abstract

A flexible system for the measurement of length and area is described. The system consists of the Reichert Jung MOP-1 area measuring device interfaced with a Commodore PET computer. Its use is illustrated by the planimetric measurement of cross sectional areas in histochemical preparations of normal and diseased muscle. While measurements are being made data can be displayed on the computer screen either in numerical form or as a frequency histogram together with simple statistical analyses. Hard copy can be obtained from an attached printer. Mean values for fibre area in normal human skeletal muscle are reported. An alternative, widely used method of calculating fibre area from the lesser diameter was found to give a consistent underestimate of approximately 30% when compared with our planimetric method. In diseased muscle with abnormally shaped fibres the discrepancy is even larger; such fibres can be identified using a "form factor" which relates the area of a cell to its perimeter. This rapid, accurate and flexible system is also suitable for the measurement of many different types of graphical record.

Citing Articles

The relationship between the morphology of nucleus of liver cells and the liver functions and prognosis of portal hypertension due to hepatic cirrhosis.

Peng Z, Tang H, Ma K, Dai Z J Tongji Med Univ. 1995; 15(2):104-7.

PMID: 8731963 DOI: 10.1007/BF02887912.

Properties of fibres, endplates and acetylcholine receptors in the diaphragm, masseter, laryngeal, abdominal and limb muscles in the goat.

Ibebunjo C, Srikant C, Donati F Can J Anaesth. 1996; 43(5 Pt 1):475-84.

PMID: 8723855 DOI: 10.1007/BF03018110.

Muscle disease, HIV and zidovudine: the spectrum of muscle disease in HIV-infected individuals treated with zidovudine.

Manji H, Harrison M, Round J, Jones D, Connolly S, Fowler C J Neurol. 1993; 240(8):479-88.

PMID: 8263554 DOI: 10.1007/BF00874117.

Measurement of striated muscle fibre diameters using interactive computer-aided microscopy.

Slavin G, Sowter C, Ward P, Paton K J Clin Pathol. 1982; 35(11):1268-71.

PMID: 7142435 PMC: 497940. DOI: 10.1136/jcp.35.11.1268.

Muscle morphology and metabolism in hypothyroid myopathy: effects of treatment.

Khaleeli A, Gohil K, McPhail G, Round J, EDWARDS R J Clin Pathol. 1983; 36(5):519-26.

PMID: 6841646 PMC: 498278. DOI: 10.1136/jcp.36.5.519.

References
1.
. Report of a sub-committee on quantitation of muscle biopsy findings. Appendix B to the Minutes of the Meeting of the Research Group on Neuromuscular Diseases, held in Montreal, Canada, on 21 September, 1967. J Neurol Sci. 1968; 6(1):179-88. View
2.
Edstrom L, Nystrom B . Histochemical types and sizes of fibres in normal human muscles. A biopsy study. Acta Neurol Scand. 1969; 45(3):257-69. DOI: 10.1111/j.1600-0404.1969.tb01238.x. View
3.
Gollnick P, Armstrong R, Saltin B, Saubert 4th C, Sembrowich W, Shepherd R . Effect of training on enzyme activity and fiber composition of human skeletal muscle. J Appl Physiol. 1973; 34(1):107-11. DOI: 10.1152/jappl.1973.34.1.107. View
4.
Edwards R, Young A, Wiles M . Needle biopsy of skeletal muscle in the diagnosis of myopathy and the clinical study of muscle function and repair. N Engl J Med. 1980; 302(5):261-71. DOI: 10.1056/NEJM198001313020504. View
5.
Round J, Matthews Y, Jones D . A quick, simple and reliable histochemical method for ATPase in human muscle preparations. Histochem J. 1980; 12(6):707-10. DOI: 10.1007/BF01012026. View