» Articles » PMID: 7044978

Enhancement of Invasiveness of Yersinia Enterocolitica and Escherichia Coli in HEp-2 Cells by Centrifugation

Overview
Journal Infect Immun
Date 1982 May 1
PMID 7044978
Citations 17
Authors
Affiliations
Soon will be listed here.
Abstract

Centrifugation enhanced the infectivity of invasive Escherichia coli and Yersinia enterocolitica for HEp-2 cells. Noninvasive bacteria were not endocytosed after centrifugation. The centrifugation procedure may increase the sensitivity of testing for bacterial invasiveness in cell culture without causing false-positive results.

Citing Articles

H nuclear magnetic resonance-based metabolite profiling of guava leaf extract: an attempt to develop a prototype for standardization of plant extracts.

Gholkar M, Li J, Daswani P, Tetali P, Birdi T BMC Complement Med Ther. 2021; 21(1):95.

PMID: 33736648 PMC: 7977270. DOI: 10.1186/s12906-021-03221-5.


Evaluation of regulated delayed attenuation strategies for Salmonella enterica serovar Typhi vaccine vectors in neonatal and infant mice.

Shi H, Wang S, Curtiss 3rd R Clin Vaccine Immunol. 2013; 20(6):931-44.

PMID: 23616408 PMC: 3675971. DOI: 10.1128/CVI.00003-13.


Newer insights into the mechanism of action of Psidium guajava L. leaves in infectious diarrhoea.

Birdi T, Daswani P, Brijesh S, Tetali P, Natu A, Antia N BMC Complement Altern Med. 2010; 10:33.

PMID: 20584265 PMC: 2911405. DOI: 10.1186/1472-6882-10-33.


Studies on the antidiarrhoeal activity of Aegle marmelos unripe fruit: validating its traditional usage.

Brijesh S, Daswani P, Tetali P, Antia N, Birdi T BMC Complement Altern Med. 2009; 9:47.

PMID: 19930633 PMC: 2788518. DOI: 10.1186/1472-6882-9-47.


Studies on Pongamia pinnata (L.) Pierre leaves: understanding the mechanism(s) of action in infectious diarrhea.

Brijesh S, Daswani P, Tetali P, Rojatkar S, Antia N, Birdi T J Zhejiang Univ Sci B. 2006; 7(8):665-74.

PMID: 16845722 PMC: 1533752. DOI: 10.1631/jzus.2006.B0665.


References
1.
Harris J, DuPont H, Hornick R . Fecal leukocytes in diarrheal illness. Ann Intern Med. 1972; 76(5):697-703. DOI: 10.7326/0003-4819-76-5-697. View

2.
Maki M, Vesikari T, Rantala I, GRONROOS P . Yersiniosis in children. Arch Dis Child. 1980; 55(11):861-5. PMC: 1626983. DOI: 10.1136/adc.55.11.861. View

3.
Devenish J, Schiemann D . HeLa cell infection by Yersinia enterocolitica: evidence for lack of intracellular multiplication and development of a new procedure for quantitative expression of infectivity. Infect Immun. 1981; 32(1):48-55. PMC: 350585. DOI: 10.1128/iai.32.1.48-55.1981. View

4.
DuPont H, Formal S, Hornick R, Snyder M, Libonati J, Sheahan D . Pathogenesis of Escherichia coli diarrhea. N Engl J Med. 1971; 285(1):1-9. DOI: 10.1056/NEJM197107012850101. View

5.
Darougar S, Cubitt S, Jones B . Effect of high-speed centrifugation on the sensitivity of irradiated McCoy cell culture for the isolation of Chlamydia. Br J Vener Dis. 1974; 50(4):308-12. PMC: 1045047. DOI: 10.1136/sti.50.4.308. View