Protein Dissociation from DNA in Model Systems and Chromatin

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1978 Sep 1

PMID 704361

Citations 2

Authors

M L Shiffman

R A Maciewicz

A W Hu

J C Howard

H J Li

Affiliations

Soon will be listed here.

Abstract

Salt induced dissociation of protamine, poly(L-lysine) and poly(L-arginine) from DNA was measured by relative light scattering at theta = 90 degrees and/or centrifugation. Dissociation of histones from DNA was studied using relative light scattering and intrinsic tyrosine fluorescence. Protamine was dissociated from DNA at 0.15 M MgCl2 (ionic strength mu = 0.45) or 0.53 M NaCl (mu = 0.53) based on light scattering data and at approximately 0.2 M MgCl2 (mu = 0.6) or 0.6 M NaCl based on centrifugation data. NaCl induced dissociation of poly(Lys) or poly(Arg) from natural DNAs measured by light scattering did not depend on the guanine plus cytosine content. To dissociate poly(Arg) from DNA higher ionic strength using NaCl, MgCl2, or CaCl2, similar ionic strength using NaClo4, and lower ionic strength using Na2SO4 was needed then to dissociated poly(Lys). Both the decrease in light scattering and the enhancement of tyrosine fluorescence of chromatin occurred between 0.5 and 1.5 M NaCl when histones were dissociated.

Citing Articles

Excitotoxic and Radiation Stress Increase TERT Levels in the Mitochondria and Cytosol of Cerebellar Purkinje Neurons.

Eitan E, Braverman C, Tichon A, Gitler D, Hutchison E, Mattson M Cerebellum. 2015; 15(4):509-17.

PMID: 26374457 PMC: 4792797. DOI: 10.1007/s12311-015-0720-6.

Salt-induced structural changes in nucleosomes.

Russev G, Vassilev L, TSANEV R Mol Biol Rep. 1980; 6(1):45-9.

PMID: 7393225 DOI: 10.1007/BF00775754.

References

Teale F, Weber G . Ultraviolet fluorescence of the aromatic amino acids. Biochem J. 1957; 65(3):476-82. PMC: 1199900. DOI: 10.1042/bj0650476. View

Weiskopf M, Li H . Poly(L-lysine)-DNA interactions in NaCl solutions: B to C and B to psi transitions. Biopolymers. 1977; 16(3):669-84. DOI: 10.1002/bip.1977.360160315. View

Polacow I, Cabasso L, Li H . Histone redistribution and conformational effect on chromatin induced by formaldehyde. Biochemistry. 1976; 15(21):4559-65. DOI: 10.1021/bi00666a002. View

Yu S, Epstein P, Li H . Helix-coil transition and conformational studies of nucleoprotein: poly(L-arginine)- and poly(L-arginine87, L-ornithine13)-DNA complexes. II. Circular dichroism. Biochemistry. 1974; 13(18):3713-7. DOI: 10.1021/bi00715a015. View

Marushige K, Bonner J . Template properties of liver chromatin. J Mol Biol. 1966; 15(1):160-74. DOI: 10.1016/s0022-2836(66)80218-8. View

Li H, Brand B, Rotter A . Thermal denaturation of calf thymus DNA: existence of a GC-richer fraction. Nucleic Acids Res. 1974; 1(2):257-65. PMC: 343344. DOI: 10.1093/nar/1.2.257. View

Leng M, Felsenfeld G . The preferential interactions of polylysine and polyarginine with specific base sequences in DNA. Proc Natl Acad Sci U S A. 1966; 56(4):1325-32. PMC: 220065. DOI: 10.1073/pnas.56.4.1325. View

Boublik M, Bradbury E, Crane-Robinson C . An investigation of the conformational changes in histones F1 and F2a1 by proton magnetic resonance spectroscopy. Eur J Biochem. 1970; 14(3):486-97. DOI: 10.1111/j.1432-1033.1970.tb00315.x. View

Subirana J . Studies on the thermal denaturation of nucleohistones. J Mol Biol. 1973; 74(3):363-86. DOI: 10.1016/0022-2836(73)90378-1. View

10.

Wickett R, Li H, Isenberg I . Salt effects on histone IV conformation. Biochemistry. 1972; 11(16):2952-7. DOI: 10.1021/bi00766a005. View

11.

Hanlon S, Johnson R, Wolf B, Chan A . Mixed conformations of deoxyribonucleic acid in chromatin: a preliminary report. Proc Natl Acad Sci U S A. 1972; 69(11):3263-7. PMC: 389750. DOI: 10.1073/pnas.69.11.3263. View

12.

Ohlenbusch H, Olivera B, Tuan D, Davidson N . Selective dissociation of histones from calf thymus nucleoprotein. J Mol Biol. 1967; 25(2):299-315. DOI: 10.1016/0022-2836(67)90143-x. View

13.

Hwan J, Leffak I, Li H, Huang P, Mura C . Studies on interaction between histone V (f2c) and deoxyribonucleic acids. Biochemistry. 1975; 14(7):1390-6. DOI: 10.1021/bi00678a008. View

14.

Santella R, Li H . Studies on poly(L-lysine50, L-tyrosine50)-DNA interaction. Biopolymers. 1974; 13(9):1909-26. DOI: 10.1002/bip.1974.360130919. View

15.

Smerdon M, Isenberg I . Conformational changes in histone GRK(f2a-1). Biochemistry. 1974; 13(19):4046-9. DOI: 10.1021/bi00716a036. View

16.

Hjelm Jr R, Huang R . The role of histones in the conformation of DNA in chromatin as studied by circular dichroism. Biochemistry. 1974; 13(26):5275-83. DOI: 10.1021/bi00723a004. View

17.

Epstein P, Yu S, Li H . Helix-coil transition and conformational studies of nucleoprotein: poly(L-arginine)- and poly(L-arginine87, L-ornithine13)-DNA complexes. I. Thermal denaturation. Biochemistry. 1974; 13(18):3706-12. DOI: 10.1021/bi00715a014. View

18.

Li H, Chang C, Weiskopf M . Helix-coil transition in nucleoprotein-chromatin structure. Biochemistry. 1973; 12(9):1763-72. DOI: 10.1021/bi00733a016. View

19.

Li H, Wickett R, Craig A, Isenberg I . Conformational changes in histone IV. Biopolymers. 1972; 11(2):375-97. DOI: 10.1002/bip.1972.360110206. View

20.

ANSEVIN A, Hnilica L, Spelsberg T, Kehm S . Structure studies on chromatin and nucleohistones. Thermal denaturation profiles recorded in the presence of urea. Biochemistry. 1971; 10(25):4793-803. DOI: 10.1021/bi00801a030. View