Iron Mediated Methylthiolation of TRNA As a Regulator of Operon Expression in Escherichia Coli

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1982 Apr 24

PMID 7043398

Citations 33

Authors

M Buck

E Griffiths

Affiliations

Soon will be listed here.

Abstract

E. coli growing in the presence of iron-binding proteins produced tRNAtrp and tRNAphe molecules containing i6A instead of ms2i6A adjacent to the anticodon. These undermodified tRNAs functioned less efficiently than the fully modified molecules when translating synthetic polynucleotides containing contiguous codons in an in vitro system, but did not limit the translation of MS2 RNA. We examined the possibility that the altered tRNAs with lowered translational efficiencies could relieve transcription termination at the trp and phe attenuators and lead to increased operon expression under iron restricted conditions. Using trpR mutants we found that there was indeed greater expression of the trp operon during iron restricted growth. This increase was attributable solely to the tRNA alteration induced by iron restriction.

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References

WETTSTEIN F, Stent G . Physiologically induced changes in the property of phenylalanine tRNA in Escherichia coli. J Mol Biol. 1968; 38(1):25-40. DOI: 10.1016/0022-2836(68)90126-5. View

Joseph D, Muench K . Tryptophanyl transfer ribonucleic acid synthetase of Escherichia coli. I. Purification of the enzyme and of tryptrophan transfer ribonucleic acid. J Biol Chem. 1971; 246(24):7602-9. View

Rose J, Yanofsky C . Metabolic regulation of the tryptophan operon of Escherichia coli: repressor-independent regulation of transcription initiation frequency. J Mol Biol. 1972; 69(1):103-18. DOI: 10.1016/0022-2836(72)90026-5. View

Agris P, Koh H, Soll D . The effect of growth temperatures on the in vivo ribose methylation of Bacillus stearothermophilus transfer RNA. Arch Biochem Biophys. 1973; 154(1):277-82. DOI: 10.1016/0003-9861(73)90058-1. View

Mann M, Huang P . Behavior of chloramphenicol-induced phenylalanine transfer ribonucleic acid during recovery from chloramphenicol treatment in Escherichia coli. Biochemistry. 1973; 12(26):5289-94. DOI: 10.1021/bi00750a011. View

Huang P, Mann M . Comparative fingerprint and composition analysis of the three forms of 32P-labeled phenylalanine tRNA from chloramphenicol-treated Escherichia coli. Biochemistry. 1974; 13(23):4704-10. DOI: 10.1021/bi00720a004. View

Juarez H, SKJOLD A, Hedgcoth C . Precursor relationship of phenylalanine transfer ribonucleic acid from Escherichia coli treated with chloramphenicol or starved for iron, methionine, or cysteine. J Bacteriol. 1975; 121(1):44-54. PMC: 285611. DOI: 10.1128/jb.121.1.44-54.1975. View

McCray Jr J, Herrmann K . Derepression of certain aromatic amino acid biosynthetic enzymes of Escherichia coli K-12 by growth in Fe3+-deficient medium. J Bacteriol. 1976; 125(2):608-15. PMC: 236122. DOI: 10.1128/jb.125.2.608-615.1976. View

Kitchingman G, Webb E, Fournier M . Unique phenylalanine transfer ribonucleic acids in relaxed control Escherichia coli: genetic origin and some functional properties. Biochemistry. 1976; 15(9):1848-57. DOI: 10.1021/bi00654a010. View

10.

Stulberg M, Sutton M, Isham K . Undermethylated transfer RNA does not support phage RNA-directed in vitro protein synthesis. Biochim Biophys Acta. 1976; 435(3):251-7. DOI: 10.1016/0005-2787(76)90106-4. View

11.

Jones C, Kearns D . Nuclear magnetic resonance of the base-pairing structure of the native and denatured conformers of Escherichia coli transfer RNATrp. J Mol Biol. 1976; 103(4):747-64. DOI: 10.1016/0022-2836(76)90207-2. View

12.

Rogers H, Synge C, Kimber B, Bayley P . Production of enterochelin by Escherichia coli 0111. Biochim Biophys Acta. 1977; 497(2):548-57. DOI: 10.1016/0304-4165(77)90211-2. View

13.

Kitchingman G, Fournier M . Modification-deficient transfer ribonucleic acids from relaxed control Escherichia coli: structures of the major undermodified phenylalanine and leucine transfer RNAs produced during leucine starvation. Biochemistry. 1977; 16(10):2213-20. DOI: 10.1021/bi00629a027. View

14.

Yanofsky C . Mutations affecting tRNATrp and its charging and their effect on regulation of transcription termination at the attenuator of the tryptophan operon. J Mol Biol. 1977; 113(4):663-77. DOI: 10.1016/0022-2836(77)90229-7. View

15.

Griffiths E, Humphreys J . Alterations in tRNAs containing 2-methylthio-N6-(delta2-isopentenyl)-adenosine during growth of enteropathogenic Escherichia coli in the presence of iron-binding proteins. Eur J Biochem. 1978; 82(2):503-13. DOI: 10.1111/j.1432-1033.1978.tb12044.x. View

16.

Lee F, Bertrand K, Bennett G, Yanofsky C . Comparison of the nucleotide sequences of the initial transcribed regions of the tryptophan operons of Escherichia coli and Salmonella typhimurium. J Mol Biol. 1978; 121(2):193-217. DOI: 10.1016/s0022-2836(78)80005-9. View

17.

Zurawski G, Brown K, Killingly D, Yanofsky C . Nucleotide sequence of the leader region of the phenylalanine operon of Escherichia coli. Proc Natl Acad Sci U S A. 1978; 75(9):4271-5. PMC: 336095. DOI: 10.1073/pnas.75.9.4271. View

18.

Laten H, Gorman J, Bock R . Isopentenyladenosine deficient tRNA from an antisuppressor mutant of Saccharomyces cerevisiae. Nucleic Acids Res. 1978; 5(11):4329-42. PMC: 342752. DOI: 10.1093/nar/5.11.4329. View

19.

Zurawski G, Elseviers D, Stauffer G, Yanofsky C . Translational control of transcription termination at the attenuator of the Escherichia coli tryptophan operon. Proc Natl Acad Sci U S A. 1978; 75(12):5988-92. PMC: 393102. DOI: 10.1073/pnas.75.12.5988. View

20.

MCCANDLISS R, Herrmann K . Iron, an essential element for biosynthesis of aromatic compounds. Proc Natl Acad Sci U S A. 1978; 75(10):4810-3. PMC: 336210. DOI: 10.1073/pnas.75.10.4810. View