» Articles » PMID: 7040482

Macrophage Origin of Reed-Sternberg Cells: an Immunohistochemical Study

Overview
Journal J Clin Pathol
Specialty Pathology
Date 1982 Feb 1
PMID 7040482
Citations 11
Authors
Affiliations
Soon will be listed here.
Abstract

In an immunohistochemical study of 26 biopsies from 24 patients with Hodgkin's disease a granular staining pattern for alpha-1-antitrypsin (alpha(1)AT) and alpha-1-antichymotrypsin (alpha(1)ACT) was seen in Reed-Sternberg (RS) cells and mononuclear Hodgkin's (H) cells in over half the cases. The pattern of staining for these antiproteases seen in RS and H cells has previously only been observed in normal and malignant cells of the monocyte/macrophage lineage within the lymphoreticular system. A faintly granular evenly distributed staining for IgG was found in viable RS and H cells. This staining was associated with a similar distribution of both light chains but not J chain, suggesting that the immunoglobulin had not been synthesised by these cells but had been taken up from the extracellular environment. It is suggested that this uptake is an active process occurring in viable RS and H cells, possibly via Fcgamma receptors and further supports an origin from cells of the monocyte/macrophage lineage. IgA, IgD, albumin, fibrinogen, C1q, C4 and C3 were present in some cells, IgM was more rarely found and lysozyme was absent. The fact that cells staining for these serum proteins generally showed signs of degeneration and that the extent of staining correlated with the molecular weight, but not serum concentration, of the protein suggests that they are passively acquired by dead or dying cells and thus represent a separate phenomenon from IgG uptake. The function of IgG uptake and accumulation by RS cells and the alpha(1)AT and alpha(1)ACT markers may prove of use in identifying the macrophage subtype from which these cells are derived.

Citing Articles

Fascin, a sensitive new marker for Reed-Sternberg cells of hodgkin's disease. Evidence for a dendritic or B cell derivation?.

Pinkus G, Pinkus J, Langhoff E, Matsumura F, Yamashiro S, Mosialos G Am J Pathol. 1997; 150(2):543-62.

PMID: 9033270 PMC: 1858289.


Characteristics of Sternberg-Reed, and related cells in Hodgkin's disease: an immunohistological study.

Dorreen M, Habeshaw J, Stansfeld A, Wrigley P, Lister T Br J Cancer. 1984; 49(4):465-76.

PMID: 6370286 PMC: 1976777. DOI: 10.1038/bjc.1984.74.


Lectin receptors as markers of lymphoid cells. II. Reed-Sternberg cells share lectin-binding properties of monocyte macrophages.

Strauchen J Am J Pathol. 1984; 116(3):370-6.

PMID: 6206728 PMC: 1900461.


Hodgkin's disease: the Sternberg-Reed cell.

Bucsky P Blut. 1987; 55(5):413-20.

PMID: 3315047 DOI: 10.1007/BF00367457.


Immunopathology of Hodgkin's disease. Characterization of Reed-Sternberg cells with monoclonal antibodies.

Strauchen J Am J Pathol. 1986; 123(2):293-300.

PMID: 3085509 PMC: 1888319.


References
1.
Leech J . Immunoglobulin-positive Reed Sternberg cells in Hodgkin's disease. Lancet. 1973; 2(7823):265-6. DOI: 10.1016/s0140-6736(73)93173-5. View

2.
Van Dalen J, Knapp W, Ploem J . Microfluorometry on antigen-antibody interaction in immunofluorescence using antigens covalently bound to agarose beads. J Immunol Methods. 1973; 2(4):383-92. DOI: 10.1016/0022-1759(73)90004-5. View

3.
DORFMAN R, Rice D, Mitchell A, Kempson R, Levine G . Ultrastructural studies of Hodgkin's disease. Natl Cancer Inst Monogr. 1973; 36:221-38. View

4.
Cohen A . Interrelationships between the human alveolar macrophage and alpha-1-antitrypsin. J Clin Invest. 1973; 52(11):2793-9. PMC: 302547. DOI: 10.1172/JCI107475. View

5.
Garvin A, SPICER S, PARMLEY R, Munster A . Immunohistochemical demonstration of IgG in Reed-Sternberg and other cells in Hodgkin's disease. J Exp Med. 1974; 139(5):1077-83. PMC: 2139657. DOI: 10.1084/jem.139.5.1077. View