Salmonella Typhimurium Mutants Defective in the Formate Dehydrogenase Linked to Nitrate Reductase

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1982 Feb 1

PMID 7035433

Citations 16

Authors

E L Barrett

D L Riggs

Affiliations

Soon will be listed here.

Abstract

Six fdn mutants of Salmonella typhimurium defective in the formation of nitrate reductase-linked formate dehydrogenase (FDHN) but capable of producing both the hydrogenase-linked formate dehydrogenase (FDHH) and nitrate reductase were characterized. Results of phage P22 transduction experiments indicated that there may be three fdn genes located on the metE-metB chromosomal segment and distinct from all previously identified fdh and chl loci. All six FDHH+ FDHN- mutants were found to make FDHN enzyme protein which was indistinguishable from that of the wild type in electrophoretic studies. However, the results of the spectral studies indicated that all six mutants were defective in the anaerobic cytochrome b559 associated with FDHN. All contained the cytochrome b559 associated with nitrate reductase in amounts equal to or greater than the wild type. The results of the transduction experiments also indicated that the metE- metB segment of the Salmonella chromosome resembles that of Escherichia coli more than was originally thought.

Citing Articles

Formate oxidation in the intestinal mucus layer enhances fitness of serovar Typhimurium.

Winter M, Hughes E, Muramatsu M, Jimenez A, Chanin R, Spiga L mBio. 2023; 14(4):e0092123.

PMID: 37498116 PMC: 10470504. DOI: 10.1128/mbio.00921-23.

Phage-mediated acquisition of a type III secreted effector protein boosts growth of salmonella by nitrate respiration.

Lopez C, Winter S, Rivera-Chavez F, Xavier M, Poon V, Nuccio S mBio. 2012; 3(3).

PMID: 22691391 PMC: 3374392. DOI: 10.1128/mBio.00143-12.

Comparative genomics of 28 Salmonella enterica isolates: evidence for CRISPR-mediated adaptive sublineage evolution.

Fricke W, Mammel M, McDermott P, Tartera C, White D, Leclerc J J Bacteriol. 2011; 193(14):3556-68.

PMID: 21602358 PMC: 3133335. DOI: 10.1128/JB.00297-11.

The hydrogenases and formate dehydrogenases of Escherichia coli.

Sawers G Antonie Van Leeuwenhoek. 1994; 66(1-3):57-88.

PMID: 7747941 DOI: 10.1007/BF00871633.

Evidence of a second nitrate reductase activity that is distinct from the respiratory enzyme in Salmonella typhimurium.

Barrett E, Riggs D J Bacteriol. 1982; 150(2):563-71.

PMID: 7040338 PMC: 216402. DOI: 10.1128/jb.150.2.563-571.1982.

References

Smith H, Levine M . A phage P22 gene controlling integration of prophage. Virology. 1967; 31(2):207-16. DOI: 10.1016/0042-6822(67)90164-x. View

Gutnick D, Calvo J, Klopotowski T, Ames B . Compounds which serve as the sole source of carbon or nitrogen for Salmonella typhimurium LT-2. J Bacteriol. 1969; 100(1):215-9. PMC: 315380. DOI: 10.1128/jb.100.1.215-219.1969. View

Ruiz-Herrera J, Demoss J . Nitrate reductase complex of Escherichia coli K-12: participation of specific formate dehydrogenase and cytochrome b1 components in nitrate reduction. J Bacteriol. 1969; 99(3):720-9. PMC: 250087. DOI: 10.1128/jb.99.3.720-729.1969. View

Stouthamer A . A genetical and biochemical study of chlorate-resistant mutants of Salmonella typhimurium. Antonie Van Leeuwenhoek. 1969; 35(4):505-21. DOI: 10.1007/BF02219168. View

Lester R, Demoss J . Effects of molybdate and selenite on formate and nitrate metabolism in Escherichia coli. J Bacteriol. 1971; 105(3):1006-14. PMC: 248530. DOI: 10.1128/jb.105.3.1006-1014.1971. View

Chippaux M, Casse F, PASCAL M . Isolation and phenotypes of mutants from Salmonella typhimurium defective in formate hydrogenlyase activity. J Bacteriol. 1972; 110(2):766-8. PMC: 247479. DOI: 10.1128/jb.110.2.766-768.1972. View

Enoch H, Lester R . Effects of molybdate, tungstate, and selenium compounds on formate dehydrogenase and other enzyme systems in Escherichia coli. J Bacteriol. 1972; 110(3):1032-40. PMC: 247525. DOI: 10.1128/jb.110.3.1032-1040.1972. View

Glaser J, Demoss J . Comparison of nitrate reductase mutants of Escherichia coli selected by alternative procedures. Mol Gen Genet. 1972; 116(1):1-10. DOI: 10.1007/BF00334254. View

Hartman P . Some improved methods in P22 transduction. Genetics. 1974; 76(4):625-31. PMC: 1213092. DOI: 10.1093/genetics/76.4.625. View

10.

MacGregor C . Synthesis of nitrate reductase components in chlorate-resistant mutants of Escherichia coli. J Bacteriol. 1975; 121(3):1117-21. PMC: 246043. DOI: 10.1128/jb.121.3.1117-1121.1975. View

11.

Enoch H, Lester R . The role of a novel cytochrome b-containing nitrate reductase and quinone in the in vitro reconstruction of formate-nitrate reductase activity of E. coli. Biochem Biophys Res Commun. 1974; 61(4):1234-41. DOI: 10.1016/s0006-291x(74)80416-x. View

12.

Enoch H, Lester R . The purification and properties of formate dehydrogenase and nitrate reductase from Escherichia coli. J Biol Chem. 1975; 250(17):6693-705. View

13.

Scott R, Demoss J . Formation of the formate-nitrate electron transport pathway from inactive components in Escherichia coli. J Bacteriol. 1976; 126(1):478-86. PMC: 233304. DOI: 10.1128/jb.126.1.478-486.1976. View

14.

Lund K, Demoss J . Association-dissociation behavior and subunit structure of heat-released nitrate reductase from Escherichia coli. J Biol Chem. 1976; 251(8):2207-16. View

15.

Bradford M . A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976; 72:248-54. DOI: 10.1016/0003-2697(76)90527-3. View

16.

Chippaux M, PASCAL M, Casse F . Formate hydrogenlyase system in Salmonella typhimurium LT2. Eur J Biochem. 1977; 72(1):149-55. DOI: 10.1111/j.1432-1033.1977.tb11234.x. View

17.

Wallace B, Young I . Role of quinones in electron transport to oxygen and nitrate in Escherichia coli. Studies with a ubiA- menA- double quinone mutant. Biochim Biophys Acta. 1977; 461(1):84-100. DOI: 10.1016/0005-2728(77)90071-8. View

18.

Demoss J . Role of the chlC gene in formation of the formate-nitrate reductase pathway in Escherichia coli. J Bacteriol. 1978; 133(2):626-30. PMC: 222068. DOI: 10.1128/jb.133.2.626-630.1978. View

19.

Sanderson K, Hartman P . Linkage map of Salmonella typhimurium, edition V. Microbiol Rev. 1978; 42(2):471-519. PMC: 281437. DOI: 10.1128/mr.42.2.471-519.1978. View

20.

Barrett E, Jackson C, Fukumoto H, Chang G . Formate dehydrogenase mutants of Salmonella typhimurium: a new medium for their isolation and new mutant classes. Mol Gen Genet. 1979; 177(1):95-101. DOI: 10.1007/BF00267258. View