Characterization of Human Muscle Myosins with Respect to the Light Chains

Overview

Journal Biochem J

Specialty Biochemistry

Date 1981 Apr 1

PMID 7030322

Citations 13

Authors

P Volpe

D Biral

E Damiani

A Margreth

Affiliations

Soon will be listed here.

Abstract

Isolated myosins from human predominantly fast and slow muscles, human neonatal and foetal muscle were examined for light chain composition by one- and two-dimensional electrophoresis. The LC1F, LC2F and LC3F light chains were identical with their counterparts from rabbit fast myosin. Human LC1S was identified by correlative criteria as a single component having a molecular weight slightly lower than, but an electric charge similar to, that of rabbit LC1Sb. Consequently, human LC1S appears to be much less heterogeneous relative to LC1F than is the case with other mammalian species. A high immunological cross-reactivity was likewise observed, with antibody specific to rabbit LC1F, between the isolated myosins from several human mixed muscles and rabbit fast myosin, though reactivity was highest with foetal myosin (having a pure-fast-light-chain pattern).

Citing Articles

Characterization study of the ryanodine receptor and of calsequestrin isoforms of mammalian skeletal muscles in relation to fibre types.

Damiani E, Margreth A J Muscle Res Cell Motil. 1994; 15(2):86-101.

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Myofibrillar-protein isoforms and sarcoplasmic-reticulum Ca2+-transport activity of single human muscle fibres.

Salviati G, Betto R, Danieli Betto D, Zeviani M Biochem J. 1984; 224(1):215-25.

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Polymorphism of sarcoplasmic-reticulum adenosine triphosphatase of rabbit skeletal muscle.

Damiani E, Betto R, Salvatori S, Volpe P, Salviati G, Margreth A Biochem J. 1981; 197(1):245-8.

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Myosin subunit composition in human developing muscle.

Biral D, Damiani E, Margreth A, Scarpini E Biochem J. 1984; 224(3):923-31.

PMID: 6395865 PMC: 1144529. DOI: 10.1042/bj2240923.

Transitions in membrane composition during postnatal development of rabbit fast muscle.

Volpe P, Damiani E, Salviati G, Margreth A J Muscle Res Cell Motil. 1982; 3(2):213-30.

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