Comparison Among Patterns of Macromolecular Synthesis in Escherichia Coli B/r at Growth Rates of Less and More Than One Doubling Per Hour at 37 Degrees C

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1981 Nov 1

PMID 7028724

Citations 7

Authors

O PIERUCCI

M Melzer

C Querini

M Rickert

C Krajewski

Affiliations

Soon will be listed here.

Abstract

In Escherichia coli B/r, the relationship between the patterns of chromosome replication and of synthesis of envelope components differs at various growth rates. At growth rates greater than 1.0 doubling per h at 37 degrees C, the average mass and age at initiation of rounds of chromosome replication are similar to those at increase in incorporation of precursors into a major outer membrane protein and phosphatidylethanolamine. At growth rates less than 1.0 doubling per h at 37 degrees C the average mass and age at increase in the synthesis of these envelope components differ from those at initiation of chromosome replication. The average cell mass per chromosomal origin at initiation of rounds of chromosome replication is not a constant and varies between growth rates greater and less than 1.0 doubling per h.

Citing Articles

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Sugai R, Shimizu H, Nishiyama K, Tokuda H J Bacteriol. 2004; 186(17):5968-71.

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Synthesis of peptidoglycan and membrane during the division cycle of rod-shaped, gram-negative bacteria.

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Duplication of Escherichia coli during inhibition of net phospholipid synthesis.

PIERUCCI O, Rickert M J Bacteriol. 1985; 162(1):374-82.

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Kepes F, Peutat L, Dari R, Kepes A J Bacteriol. 1987; 169(8):3701-6.

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References

SHOCKMAN G, Higgins M . Problems of cell wall and membrane growth, enlargement, and division. Ann N Y Acad Sci. 1974; 235(0):161-97. DOI: 10.1111/j.1749-6632.1974.tb43265.x. View

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

Takacs B, Rosenbusch J . Modification of Escherichia coli membranes in the prereplicative phase of phage T4 infection. Specificity of association and quantitation of bound phage proteins. J Biol Chem. 1975; 250(6):2339-50. View

KUBITSCHEK H . Estimation of the D period from residual division after exposure of exponential phase bacteria to chloramphenicol. Mol Gen Genet. 1974; 135(2):123-30. DOI: 10.1007/BF00264780. View

Weber K, Pringle J, Osborn M . Measurement of molecular weights by electrophoresis on SDS-acrylamide gel. Methods Enzymol. 1972; 26:3-27. DOI: 10.1016/s0076-6879(72)26003-7. View

Cooper S, Helmstetter C . Chromosome replication and the division cycle of Escherichia coli B/r. J Mol Biol. 1968; 31(3):519-40. DOI: 10.1016/0022-2836(68)90425-7. View

Cronan Jr J . Phospholipid alterations during growth of Escherichia coli. J Bacteriol. 1968; 95(6):2054-61. PMC: 315134. DOI: 10.1128/jb.95.6.2054-2061.1968. View

DONACHIE W . Relationship between cell size and time of initiation of DNA replication. Nature. 1968; 219(5158):1077-9. DOI: 10.1038/2191077a0. View

Cooper S . Cell division and DNA replication following a shift to a richer medium. J Mol Biol. 1969; 43(1):1-11. DOI: 10.1016/0022-2836(69)90074-6. View

10.

Schnaitman C . Effect of ethylenediaminetetraacetic acid, Triton X-100, and lysozyme on the morphology and chemical composition of isolate cell walls of Escherichia coli. J Bacteriol. 1971; 108(1):553-63. PMC: 247097. DOI: 10.1128/jb.108.1.553-563.1971. View

11.

PIERUCCI O . Chromosome replication and cell division in Escherichia coli at various temperatures of growth. J Bacteriol. 1972; 109(2):848-54. PMC: 285215. DOI: 10.1128/jb.109.2.848-854.1972. View

12.

Zusman D, Inouye M, Pardee A . Cell division in Escherichia coli: evidence for regulation of septation by effector molecules. J Mol Biol. 1972; 69(1):119-36. DOI: 10.1016/0022-2836(72)90027-7. View

13.

Hoffmann B, Messer W, Schwarz U . Regulation of polar cap formation in the life cycle of Escherichia coli. J Supramol Struct. 1972; 1(1):29-37. DOI: 10.1002/jss.400010105. View

14.

Zaritsky A, Pritchard R . Changes in cell size and shape associated with changes in the replication time of the chromosome of Escherichia coli. J Bacteriol. 1973; 114(2):824-37. PMC: 251845. DOI: 10.1128/jb.114.2.824-837.1973. View

15.

Jones N, DONACHIE W . Chromosome replication, transcription and control of cell division in Escherichia coli. Nat New Biol. 1973; 243(125):100-3. View

16.

Hirashima A, Childs G, Inouye M . Differential inhibitory effects of antibiotics on the biosynthesis of envelope proteins of Escherichia coli. J Mol Biol. 1973; 79(2):373-89. DOI: 10.1016/0022-2836(73)90012-0. View

17.

James R, Haga J, Pardee A . Inhibition of an early event in the cell division cycle of Escherichia coli by FL1060, an amidinopenicillanic acid. J Bacteriol. 1975; 122(3):1283-92. PMC: 246186. DOI: 10.1128/jb.122.3.1283-1292.1975. View

18.

James R, Gudas L . Cell cycle-specific incorporation of lipoprotein into the outer membrane of Escherichia coli. J Bacteriol. 1976; 125(1):374-5. PMC: 233375. DOI: 10.1128/jb.125.1.374-375.1976. View

19.

Taylor F, Cronan Jr J . Selection and properties of Escherichia coli mutants defective in the synthesis of cyclopropane fatty acids. J Bacteriol. 1976; 125(2):518-23. PMC: 236110. DOI: 10.1128/jb.125.2.518-523.1976. View

20.

Zaritsky A . On dimensional determination of rod-shaped bacteria. J Theor Biol. 1975; 54(2):243-8. DOI: 10.1016/s0022-5193(75)80129-9. View