Immunohistochemical Localization of Cholinergic Nerve Terminals

Overview

Journal Cell Tissue Res

Specialties Anatomy & Histology
Cell Biology

Date 1981 Jan 1

PMID 7020949

Citations 14

Authors

R T Jones

J H Walker

P J Richardson

G Q Fox

V P Whittaker

Affiliations

Soon will be listed here.

Abstract

Most of the published light-microscopic methods for the localization of cholinergic nerve pathways present various difficulties of interpretation. The production and characterization of an antiserum that binds specifically to cholinergic terminals is described. The antiserum was raised to small synaptosomes prepared from the purely cholinergic electric organ of Torpedo marmorata. It was shown to lyse cholinergic synaptosomes in a mixed population derived from guinea-pig cortex. After partial purification by adsorption onto nonspecific antigens, It was used to label nerve endings in several tissues of Torpedo, rat and guinea pigs using indirect immunofluorescence histochemistry. The antiserum appears to provide a highly specific means of localizing cholinergic nerve endings in these tissues.

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References

Fonnum F . A radiochemical method for the estimation of choline acetyltransferase. Biochem J. 1966; 100(2):479-84. PMC: 1265159. DOI: 10.1042/bj1000479. View

HOOPER J, Carlson S, Kelly R . Antibodies to synaptic vesicles purified from Narcine electric organ bind a subclass of mammalian nerve terminals. J Cell Biol. 1980; 87(1):104-13. PMC: 2110736. DOI: 10.1083/jcb.87.1.104. View

Whittaker V . The electromotor system of Torpedo. A model cholinergic system. Naturwissenschaften. 1977; 64(12):606-11. DOI: 10.1007/BF00440092. View

Willingham M, Yamada S, Pastan I . Ultrastructural antibody localization of alpha2-macroglobulin in membrane-limited vesicles in cultured cells. Proc Natl Acad Sci U S A. 1978; 75(9):4359-63. PMC: 336114. DOI: 10.1073/pnas.75.9.4359. View

Kuhar M, Rommelspacher H . Acetylcholinesterase-staining synaptosomes from rat hippocampus: relative frequency and tentative estimation of internal concentration of free or 'labile bound' acetylcholine. Brain Res. 1974; 77(1):85-96. DOI: 10.1016/0006-8993(74)90806-3. View

Karnovsky M, ROOTS L . A "DIRECT-COLORING" THIOCHOLINE METHOD FOR CHOLINESTERASES. J Histochem Cytochem. 1964; 12:219-21. DOI: 10.1177/12.3.219. View

Lewis P, SHUTE C, Silver A . Confirmation from choline acetylase analyses of a massive cholinergic innervation to the rat hippocampus. J Physiol. 1967; 191(1):215-24. PMC: 1365448. DOI: 10.1113/jphysiol.1967.sp008246. View

Iversen L, Bloom F . Studies of the uptake of 3 H-gaba and ( 3 H)glycine in slices and homogenates of rat brain and spinal cord by electron microscopic autoradiography. Brain Res. 1972; 41(1):131-43. DOI: 10.1016/0006-8993(72)90621-x. View

Gray E, Whittaker V . The isolation of nerve endings from brain: an electron-microscopic study of cell fragments derived by homogenization and centrifugation. J Anat. 1962; 96:79-88. PMC: 1244174. View

10.

Eng L, UYEDA C, Chao L, WOLFGRAM F . Antibody to bovine choline acetyltransferase and immunofluorescent localisation of the enzyme in neurones. Nature. 1974; 250(463):243-5. DOI: 10.1038/250243a0. View

11.

Storm-Mathisen J . Localization of transmitter candidates in the brain: the hippocampal formation as a model. Prog Neurobiol. 1977; 8(2):119-81. DOI: 10.1016/0301-0082(77)90013-2. View

12.

Black I, Bloom F, Hendry I, Iversen L . Growth and development of sympathetic ganglion: maturation of transmitter enzymes and synapse formation in the mouse superior cervical ganglion. J Physiol. 1971; 215(1):24P-25P. View

13.

McLaughlin B, Wood J, Saito K, Barber R, Vaughn J, Roberts E . The fine structural localization of glutamate decarboxylase in synaptic terminals of rodent cerebellum. Brain Res. 1974; 76(3):377-91. DOI: 10.1016/0006-8993(74)90815-4. View

14.

Johnson M . The intracellular distribution of glycolytic and other enzymes in rat-brain homogenates and mitochondrial preparations. Biochem J. 1960; 77:610-8. PMC: 1205083. DOI: 10.1042/bj0770610. View

15.

Gabella G . Innervation of the gastrointestinal tract. Int Rev Cytol. 1979; 59:129-93. DOI: 10.1016/s0074-7696(08)61662-9. View

16.

SHERIDAN M . THE FINE STRUCTURE OF THE ELECTRIC ORGAN OF TORPEDO MARMORATA. J Cell Biol. 1965; 24:129-41. PMC: 2106563. DOI: 10.1083/jcb.24.1.129. View

17.

Carlson S, Kelly R . An antiserum specific for cholinergic synaptic vesicles from electric organ. J Cell Biol. 1980; 87(1):98-103. PMC: 2110719. DOI: 10.1083/jcb.87.1.98. View

18.

Stadler H, Tashiro T . Isolation of synaptosomal plasma membranes from cholinergic nerve terminals and a comparison of their proteins with those of synaptic vesicles. Eur J Biochem. 1979; 101(1):171-8. DOI: 10.1111/j.1432-1033.1979.tb04229.x. View

19.

SHUTE C, Lewis P . Electron microscopy of cholinergic terminals and acetylcholinesterase-containing neurones in the hippocampal formation of the rat. Z Zellforsch Mikrosk Anat. 1966; 69:334-43. DOI: 10.1007/BF00406286. View

20.

Gunn M . Histological and histochemical observations on the myenteric and submucous plexuses of mammals. J Anat. 1968; 102(Pt 2):223-39. PMC: 1231312. View