Long-term Culture of Epithelial Cells from the Normal Rat Colon

Overview

Journal In Vitro

Specialties Anatomy & Histology
Cell Biology

Date 1981 May 1

PMID 7019042

Citations 9

Authors

D P Chopra

K Y Yeh

Affiliations

Soon will be listed here.

Abstract

Serial passage cultures of colonic epithelial cells from young rats have been maintained for more than 6 months in Eagle's minimum essential medium buffered with HEPES (25 mM) and supplemented with 2.5% fetal bovine serum, 0.5 micrograms/ml insulin, 5.0 micrograms/ml transferrin, and antibiotics. The cells proliferated in this medium with a population doubling time of approximately 53 h. The cells retained differentiated morphology as evidenced by secretory activity and the presence of secretory granules, microvilli, tonofilaments, and desmosomal junctions. Further, cells at the fourth passage had normal karyotypes with 42 chromosomes and exhibited anchorage dependent growth. High concentrations of fetal bovine serum (10 to 15%) exerted toxic effects on the colonic epithelial cell cultures.

Citing Articles

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