The Utilization of Native Glioma Antigens in the Assessment of Cellular and Humoral Immune Responses in Malignant Glioma Patients

Overview

Journal Acta Neurochir (Wien)

Specialty Neurosurgery

Date 1981 Jan 1

PMID 7015801

Citations 1

Authors

M L Apuzzo

K M Sheikh

M H WEISS

J S Heiden

T Kurze

Affiliations

Soon will be listed here.

Abstract

Cellular and humoral components of the immune response have been studied preoperatively, concurrently, and serially in patients with malignant glial neoplasms. In order to assess titres of circulating antibodies to tumour cell constituents an indirect immunofluorescent technique was applied to single cell suspensions and snap frozen cell smears. In an allogeneic system, 49% of 47 test and 7% of 124 control sera gave a positive response to cytoplasmic components. The leucocyte adherence inhibition assay was applied to study 39 test and 64 control patients. Significant non-adherence of leukocytes was observed in 77% of test cases. Control parameters indicated specificity of the response. Simultaneous assessment in 28 test patients yielded a positive response for one or both assays in 89% of cases..

Citing Articles

Electron-microscopic visualization of binding of antibodies from sera of glioma patients on cultured glioma cells.

Lauro G, Solheid C, Di Lorenzo N, GUIDETTI B Acta Neuropathol. 1983; 59(2):127-32.

PMID: 6837274 DOI: 10.1007/BF00691598.

References

Boker D . [Immunfluorescence of human brain tumours (author's transl)]. Acta Neurochir (Wien). 1978; 41(4):363-71. DOI: 10.1007/BF01811349. View

Holt P, Fimmel P, Flower R . Evaluation of the microplate leukocyte adherence inhibition test and its reproducibility, sensitivity, and relationship to other tests of cellular immunity. Cancer Res. 1979; 39(2 Pt 2):564-9. View

Meltzer M, Leonard E, Rapp H, Borsos T . Tumor-specific antigen solubilized by hypertonic potassium chloride. J Natl Cancer Inst. 1971; 47(3):703-9. View

Coakham H, Lakshmi M . Tumour-associated surface antigen(s) in human astrocytomas. Oncology. 1975; 31(3-4):234-43. View

Apuzzo M, Sheikh K, Heiden J, WEISS M, Kurze T . Definition of cellular immune responses to brain antigens in human head trauma. J Neurosurg. 1979; 51(3):317-22. DOI: 10.3171/jns.1979.51.3.0317. View

Solheid C, Lauro G, Palladini G . Two separate membrane-bound antigens on human glioma cells in tissue culture detected with sera from glioma patients by immunofluorescence. J Neurol Sci. 1976; 30(1):55-64. DOI: 10.1016/0022-510x(76)90255-0. View

Grosser N, Marti J, Proctor J, Thomson D . Tube leukocyte adherence inhibition assay for the detection of anti-tumor immunity. I. Monocyte is the reactive cell. Int J Cancer. 1976; 18(1):39-47. DOI: 10.1002/ijc.2910180107. View

Trachtenberg M, KORNBLITH P, Hauptli J . Biophysical properties of cultured human glial cells. Brain Res. 1972; 38(2):279-98. DOI: 10.1016/0006-8993(72)90713-5. View

Tataryn D, Macfarlane J, Thomson D . Leucocyte adherence inhibition for detecting specific tumour immunity in early pancreatic cancer. Lancet. 1978; 1(8072):1020-2. DOI: 10.1016/s0140-6736(78)90742-0. View

10.

Quindlen E, DOHAN Jr F, KORNBLITH P . Improved assay for cytotoxic antiglioma antibody. Surg Forum. 1974; 25(0):464-6. View

11.

Kumar S, Taylor G . Specific lymphocytotoxicity and blocking factors in tumours of the central nervous system. Br J Cancer Suppl. 1973; 1:135-41. PMC: 2149049. View

12.

Thomas D, Lannigan C, Behan P . Letter: Impaired cell-mediated immunity in human brain tumours. Lancet. 1975; 1(7921):1389-90. DOI: 10.1016/s0140-6736(75)92308-9. View

13.

Pees H . Cytotoxic immune response of meningioma patients towards allogeneic and autologous tumour cells before and after surgery. Acta Neurochir (Wien). 1979; 50(3-4):217-27. DOI: 10.1007/BF01808518. View

14.

Levy N, MAHALEY Jr M, DAY E . In vitro demonstration of cell-mediated immunity to human brain tumors. Cancer Res. 1972; 32(3):477-82. View

15.

Mottet S, Tribolet N, Diserens A . Evaluation of the humoral response of meningioma patients to possible common tumour-associated antigens. Acta Neurochir (Wien). 1980; 52(1-2):19-25. DOI: 10.1007/BF01400942. View

16.

Marti J, Thomson D . Anti-tumour immunity in malignant melanoma assay by tube leucocyte adherence inhibition. Br J Cancer. 1976; 34(2):116-33. PMC: 2025156. DOI: 10.1038/bjc.1976.134. View

17.

MAHALEY Jr M, Brooks W, Roszman T, Bigner D, Dudka L, Richardson S . Immunobiology of primary intracranial tumors. Part 1: studies of the cellular and humoral general immune competence of brain-tumor patients. J Neurosurg. 1977; 46(4):467-76. DOI: 10.3171/jns.1977.46.4.0467. View

18.

RODA J, Villarejo F, Heredero J, OBRADOR S . Heterologous antibodies in human and rat's glioblastoma. Acta Neurochir (Wien). 1976; 35(1-3):53-6. DOI: 10.1007/BF01405932. View

19.

Brooks W, NETSKY M, Normansell D, Horwitz D . Depressed cell-mediated immunity in patients with primary intracranial tumors. Characterization of a humoral immunosuppressive factor. J Exp Med. 1972; 136(6):1631-47. PMC: 2139329. DOI: 10.1084/jem.136.6.1631. View

20.

MAHALEY Jr M . Immunotherapy of brain tumors. Semin Oncol. 1975; 2(1):75-8. View