Immunoperoxidase Localisation of Human Placental Lactogen: a Marker for the Placental Origin of the Giant Cells in 'syncytial Endometritis' of Pregnancy

Overview

Journal J Clin Pathol

Specialty Pathology

Date 1981 Mar 1

PMID 7014653

Citations 2

Authors

E Heyderman

A R Gibbons

S W ROSEN

Affiliations

Soon will be listed here.

Abstract

One hundred endometrial biopsies of various histological patterns, and material from 10 tubal pregnancies together with their associated uterine decidua, were examined for the presence of human placental lactogen using affinity-purified first and second antibodies and an indirect immunoperoxidase technique. Positive cells in endometrial curettings were seen only in association with an intrauterine pregnancy and morphologically resembled syncytiotrophoblast. Decidua associated with tubal pregnancy, pseudodecidua in progestogen-treated patients, and proliferative, secretory, and basal endometria were all negative. An immunoperoxidase stain for human placental lactogen is a useful marker for intrauterine pregnancy and supports the placental origin of the syncytial giant cells in so-called 'syncytial endometritis'. The technique is of potential value in those endometrial biopsies where pregnancy is suspected but no villi are seen.

Citing Articles

Epithelial markers in prostatic, bladder, and colorectal cancer: an immunoperoxidase study of epithelial membrane antigen, carcinoembryonic antigen, and prostatic acid phosphatase.

Heyderman E, Brown B, Richardson T J Clin Pathol. 1984; 37(12):1363-9.

PMID: 6392348 PMC: 499028. DOI: 10.1136/jcp.37.12.1363.

Immunolocalization of alpha and beta chains of human chorionic gonadotropin, placental lactogen and pregnancy-specific beta-glycoprotein in term placenta by a touch preparation method.

Morrish D, Manickavel V, Jewell L, Siy O Histochemistry. 1987; 88(1):57-60.

PMID: 2449404 DOI: 10.1007/BF00490168.

References

Currie A, BECK J, Ellis S, READ C . Immunofluorescent localisation of a growth hormone-like factor in normal and abnormal syncytiotrophoblast. J Pathol Bacteriol. 1966; 92(2):395-9. DOI: 10.1002/path.1700920217. View

Boyd J, HAMILTON W . The giant cells of the pregnant human uterus. J Obstet Gynaecol Br Emp. 1960; 67:208-18. DOI: 10.1111/j.1471-0528.1960.tb06980.x. View

Weintraub B, ROSEN S . Ectopic production of human chorionic somatomammotropin by nontrophoblastic cancers. J Clin Endocrinol Metab. 1971; 32(1):94-101. DOI: 10.1210/jcem-32-1-94. View

Morgan F, Canfield R . Nature of the subunits of human chorionic gonadotropin. Endocrinology. 1971; 88(4):1045-53. DOI: 10.1210/endo-88-4-1045. View

Teoh E, Spellacy W, Buhi W . Human chorionic somatomammotrophin (HCS): a new index of placental function. J Obstet Gynaecol Br Commonw. 1971; 78(8):673-85. DOI: 10.1111/j.1471-0528.1971.tb01628.x. View

Bohn H . [Detection and characterization of pregnancy proteins in the human placenta and their quantitative immunochemical determination in sera from pregnant women]. Arch Gynakol. 1971; 210(4):440-57. DOI: 10.1007/BF01628222. View

Payne R, RYAN R . Human placental lactogen in the male subject. J Urol. 1972; 107(1):99-103. DOI: 10.1016/s0022-5347(17)60958-3. View

Elston C, Bagshawe K . The diagnosis of trophoblastic tumours from uterine curettings. J Clin Pathol. 1972; 25(2):111-8. PMC: 477238. View

Vaitukaitis J, Braunstein G, ROSS G . A radioimmunoassay which specifically measures human chorionic gonadotropin in the presence of human luteinizing hormone. Am J Obstet Gynecol. 1972; 113(6):751-8. DOI: 10.1016/0002-9378(72)90553-4. View

10.

de Wolf F, De Wolf-Peeters C, Brosens I . Ultrastructure of the spiral arteries in the human placental bed at the end of normal pregnancy. Am J Obstet Gynecol. 1973; 117(6):833-48. DOI: 10.1016/0002-9378(73)90500-0. View

11.

Niall H, Hogan M, Tregear G, Segre G, Hwang P, Friesen H . The chemistry of growth hormone and the lactogenic hormones. Recent Prog Horm Res. 1973; 29:387-416. DOI: 10.1016/b978-0-12-571129-6.50014-2. View

12.

Robertson W, Warner B . The ultrastructure of the human placental bed. J Pathol. 1974; 112(4):203-11. DOI: 10.1002/path.1711120403. View

13.

Nakane P, Kawaoi A . Peroxidase-labeled antibody. A new method of conjugation. J Histochem Cytochem. 1974; 22(12):1084-91. DOI: 10.1177/22.12.1084. View

14.

Heyderman E, Neville A . Letter: Syncytiotrophoblasts in malignant testicular tumours. Lancet. 1976; 2(7976):103. DOI: 10.1016/s0140-6736(76)92325-4. View

15.

Horne C, Reid I, Milne G . Prognostic significance of inappropriate production of pregnancy proteins by breast cancers. Lancet. 1976; 2(7980):279-82. DOI: 10.1016/s0140-6736(76)90731-5. View

16.

Kurman R, Scully R, NORRIS H . Trophoblastic pseudotumor of the uterus: an exaggerated form of "syncytial endometritis" simulating a malignant tumor. Cancer. 1976; 38(3):1214-26. DOI: 10.1002/1097-0142(197609)38:3<1214::aid-cncr2820380323>3.0.co;2-j. View

17.

Riddick D, Kusmik W . Decidua: a possible source of amniotic fluid prolactin. Am J Obstet Gynecol. 1977; 127(2):187-90. DOI: 10.1016/s0002-9378(16)33248-3. View

18.

Horne C, Towler C, Milne G . Detection of pregnancy specific beta1-glycoprotein in formalin-fixed tissues. J Clin Pathol. 1977; 30(1):19-23. PMC: 476625. DOI: 10.1136/jcp.30.1.19. View

19.

Heyderman E, Neville A . A shorter immunoperoxidase technique for the demonstration of carcinoembryonic antigen and other cell products. J Clin Pathol. 1977; 30(2):138-40. PMC: 476336. DOI: 10.1136/jcp.30.2.138. View

20.

Watkins W . Use of immunocytochemical techniques for the localization of human placental lactogen. J Histochem Cytochem. 1978; 26(4):288-92. DOI: 10.1177/26.4.96175. View