An Immunocytochemical Investigation of Non-neuronal Enolase in Cerebellum: a New Astrocyte Marker

Overview

Journal Histochem J

Specialty Biochemistry

Date 1981 Jan 1

PMID 7014537

Citations 3

Authors

O K Langley

M S Ghandour

Affiliations

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Abstract

Immunohistochemical methods were used to study in the optical and electron microscopes the localization of the so-called 'non-neuronal enolase' (the alpha apha-isoenzyme) in adult mouse cerebellum. Three separate methods, including a novel modification involving sequential incubation with biotin-conjugated sheep anti-rabbit serum and avidin-conjugated peroxidase to reveal tissue-bound specific antibody, gave similar results. The enolase was found exclusively in astrocytes: astrocyte perikaryal cytoplasm and processes were heavily labelled and nuclei were frequently stained. The extensive network of astrocyte processes in the granular layer and white matter and the Bergmann fibres in the molecular layer are readily visualized by these methods. Neither neurons nor oligodendrocytes were found to be labelled. The results are discussed in relation to the possible existence of a hybrid form of the enolase and the biochemistry of cerebellar glial cells.

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References

Schmechel D, Marangos P, Zis A, Brightman M, Goodwin F . Brain endolases as specific markers of neuronal and glial cells. Science. 1978; 199(4326):313-5. DOI: 10.1126/science.339349. View

Ghandour M, Langley O, Labourdette G, Vincendon G, Gombos G . Specific and artefactual cellular localizations of S 100 protein: an astrocyte marker in rat cerebellum. Dev Neurosci. 1981; 4(1):66-78. DOI: 10.1159/000112742. View

Moore B, McGregor D . CHROMATOGRAPHIC AND ELECTROPHORETIC FRACTIONATION OF SOLUBLE PROTEINS OF BRAIN AND LIVER. J Biol Chem. 1965; 240:1647-53. View

Fletcher L, Rider C, Taylor C . Enolase isoenzymes. III. Chromatographic and immunological characteristics of rat brain enolase. Biochim Biophys Acta. 1976; 452(1):245-52. DOI: 10.1016/0005-2744(76)90077-2. View

STERNBERGER L . The unlabeled antibody (PAP) method, introduction. J Histochem Cytochem. 1979; 27(12):1657. DOI: 10.1177/27.12.392001. View

Pickel V, Reis D, Marangos P, Zomzely-Neurath C . Immunocytochemical localization of nervous system specific protein (NSP-R) in rat brain. Brain Res. 1976; 105(1):184-7. DOI: 10.1016/0006-8993(76)90936-7. View

Ghandour M, Langley O, Vincendon G, Gombos G, Filippi D, LIMOZIN N . Immunochemical and immunohistochemical study of carbonic anhydrase II in adult rat cerebellum: a marker for oligodendrocytes. Neuroscience. 1980; 5(3):559-71. DOI: 10.1016/0306-4522(80)90053-6. View

Ghandour M, Langley O, Vincendon G, Gombos G . Double labeling immunohistochemical technique provides evidence of the specificity of glial cell markers. J Histochem Cytochem. 1979; 27(12):1634-7. DOI: 10.1177/27.12.118210. View

Baranowski T, WOLNA E . Enolase from human muscle. Methods Enzymol. 1975; 42:335-8. DOI: 10.1016/0076-6879(75)42137-1. View

10.

Marangos P, Schmechel D, Zis A, Goodwin F . The existence and neurobiological significance of neuronal and glial forms of the glycolytic enzyme enolase. Biol Psychiatry. 1979; 14(4):563-79. View

11.

MATUS A, Mughal S . Immunohistochemical localisation of S-100 protein in brain. Nature. 1975; 258(5537):746-8. DOI: 10.1038/258746a0. View

12.

Schachner M, Hedley-Whyte E, Hsu D, Schoonmaker G, Bignami A . Ultrastructural localization of glial fibrillary acidic protein in mouse cerebellum by immunoperoxidase labeling. J Cell Biol. 1977; 75(1):67-73. PMC: 2111556. DOI: 10.1083/jcb.75.1.67. View

13.

Rider C, Taylor C . Enolase isoenzymes in rat tissues. Electrophoretic, chromatographic, immunological and kinetic properties. Biochim Biophys Acta. 1974; 365(1):285-300. DOI: 10.1016/0005-2795(74)90273-6. View

14.

Rider C, Taylor C . Evidence for a new form of enolase in rat brain. Biochem Biophys Res Commun. 1975; 66(2):814-20. DOI: 10.1016/0006-291x(75)90582-3. View

15.

Ghandour M, Langley O, Keller A . A comparative immunohistological study of cerebellar enolases. Double labelling technique and immunoelectronmicroscopy. Exp Brain Res. 1981; 41(3-4):271-9. DOI: 10.1007/BF00238884. View

16.

Marangos P, Zomzely-Neurath C, YORK C . Determination and characterization of neuron specific protein (NSP) associated enolase activity. Biochem Biophys Res Commun. 1976; 68(4):1309-16. DOI: 10.1016/0006-291x(76)90339-9. View

17.

Bock E . The nature of the two proteins of brain specific antigen 14-3-2. J Neurochem. 1978; 30(1):181-5. DOI: 10.1111/j.1471-4159.1978.tb07050.x. View

18.

Marangos P, Zis A, CLARK R, Goodwin F . Neuronal, non-neuronal and hybrid forms of enolase in brain: structural, immunological and functional comparisons. Brain Res. 1978; 150(1):117-33. DOI: 10.1016/0006-8993(78)90657-1. View

19.

Langley O, Ghandour M, Vincendon G, Gombos G . An ultrastructural immunocytochemical study of nerve-specific protein in rat cerebellum. J Neurocytol. 1980; 9(6):783-98. DOI: 10.1007/BF01205019. View