Cadmium-binding Component in Escherichia Coli During Accommodation to Low Levels of This Ion

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 1981 Jan 1

PMID 7013701

Citations 15

Authors

M B Khazaeli

R S Mitra

Affiliations

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Abstract

An inducible cadmium-binding protein was isolated from Escherichia coli cells accommodated to 3 X 10(-6) M Cd2+ but not from normal or unaccommodated cells. Sephadex G-100, metal chelate affinity chromatography, and disc gel electrophoresis were used in the purification procedure. The molecular weight of the Cd2+-binding protein was estimated to be about 39,000 by Sephadex G-100 chromatography, making it different from the conventional, much smaller metallothionein.

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