The Influence of Colicinogenic Plasmids ColIb-P9, ColIa-CA53 and ColV-K30 on the Repair, Mutagenesis and Induction of Colicin E1 Synthesis

Overview

Journal Mol Gen Genet

Specialties Genetics
Molecular Biology

Date 1981 Jan 1

PMID 7012544

Citations 4

Authors

I A Khmel

V M Kopylov

I P Vorobjeva

V P Polyanin

Affiliations

Soon will be listed here.

Abstract

The presence of colicinogenic plasmids ColIb-P9 and ColIa-CA53 in E. coli K-12 cells, wild-type with respect to repair, enhanced the survival of cells after UV irradiation and increased the frequency of UV-induced argE3 and his-4 reversions, while the presence of ColV-K30 negatively affected repair and mutagenesis. The plasmid ColIb-P9 showed a UV-protective effect in E. coli cells carrying mutations in genes uvrA, uvrB, uvrC, polA, recB, recF, though in none of the mutants did cell survival reach the wild-type level. The effect of ColIb-P9 on mutagenesis did not depend on the uvrA or recB genes. The plasmid's protective effect and the enhancement of mutagenesis depended on the recA+ lexA+ genotype. The frequency of 2-aminopurine-induced mutations was not affected by ColIb-P9 or ColV-K30. The presence of ColIb-P9 decreased the ability of ColE1-carrying cells to induce colicin E1 synthesis caused by DNA-damaging agents: UV, MNNG, mitomycin C, whereas ColV-K30 increased the percentage of colicin E1-producing cells. These plasmid effects on the level of induction of colicin E1 synthesis were not observed in the case of induction caused by chloramphenicol which did not depend on the products of recA and lexA genes.

Citing Articles

Microcin C51 plasmid genes: possible source of horizontal gene transfer.

Fomenko D, Metlitskaya A, Peduzzi J, Goulard C, Katrukha G, Gening L Antimicrob Agents Chemother. 2003; 47(9):2868-74.

PMID: 12936987 PMC: 182647. DOI: 10.1128/AAC.47.9.2868-2874.2003.

Cloning and mapping of the genetic determinants for microcin C51 production and immunity.

Kurepina N, Basyuk E, Metlitskaya A, Zaitsev D, Khmel I Mol Gen Genet. 1993; 241(5-6):700-6.

PMID: 8264544 DOI: 10.1007/BF00279914.

Autoinduced synthesis of colicin E2.

Pugsley A Mol Gen Genet. 1983; 190(3):379-83.

PMID: 6348478 DOI: 10.1007/BF00331062.

Effect of the plasmid ColIb-P9 on cellular processes related to DNA repair.

Kopylov V, Khmel I, Vorobjeva I, Lipasova V, Kolot M Mol Gen Genet. 1984; 193(3):520-4.

PMID: 6323923 DOI: 10.1007/BF00382093.

References

Mortelmans K, Stocker B . Ultraviolet light protection, enhancement of ultraviolet light mutagenesis, and mutator effect of plasmid R46 in Salmonella typhimurium. J Bacteriol. 1976; 128(1):271-82. PMC: 232853. DOI: 10.1128/jb.128.1.271-282.1976. View

Walker G . Inducible reactivation and mutagenesis of UV-irradiated bacteriophage P22 in Salmonella typhimurium LT2 containing the plasmid pKM101. J Bacteriol. 1978; 135(2):415-21. PMC: 222398. DOI: 10.1128/jb.135.2.415-421.1978. View

WITKIN E . Ultraviolet mutagenesis and inducible DNA repair in Escherichia coli. Bacteriol Rev. 1976; 40(4):869-907. PMC: 413988. DOI: 10.1128/br.40.4.869-907.1976. View

Clewell D, Helinski D . Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form. Proc Natl Acad Sci U S A. 1969; 62(4):1159-66. PMC: 223628. DOI: 10.1073/pnas.62.4.1159. View

Howarth S . Increase in frequency of ultraviolet-induced mutation brought about by the colicine factor, col-I in Salmonella typhimurium. Mutat Res. 1966; 3(2):129-34. DOI: 10.1016/0027-5107(66)90026-1. View

BOGDANOVA S, Fetisova I, Gavrilov V . [Induction of colicin E-1 synthesis by bruneomycin and N-methyl-N'-nitro-N-nitrosoguanidine]. Mikrobiologiia. 1970; 39(4):622-5. View

HOWARD-FLANDERS P, Boyce R . DNA repair and genetic recombination: studies on mutants of Escherichia coli defective in these processes. Radiat Res. 1966; :Suppl 6:156+. View

Walker G . Plasmid (pKM101)-mediated enhancement of repair and mutagenesis: dependence on chromosomal genes in Escherichia coli K-12. Mol Gen Genet. 1977; 152(1):93-103. DOI: 10.1007/BF00264945. View

Monti-Bragadin C, Babudri N, Samer L . Expression of the plasmid pKM101--determined DNA repair system in recA- and lex- strains of Escherichia coli. Mol Gen Genet. 1976; 145(3):303-6. DOI: 10.1007/BF00325827. View

10.

Cohen S, Chang A, Hsu L . Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA. Proc Natl Acad Sci U S A. 1972; 69(8):2110-4. PMC: 426879. DOI: 10.1073/pnas.69.8.2110. View

11.

MacPhee D . Effect of an R factor on resistance of Salmonella typhimurium to radiation and chemical treatment. Mutat Res. 1972; 14(4):450-3. DOI: 10.1016/0027-5107(72)90146-7. View

12.

Smith S, Ozeki H, Stocker B . TRANSFER OF COLE1 AND COLE2 DURING HIGH-FREQUENCY TRANSMISSION OF COLI IN SALMONELLA TYPHIMURIUM. J Gen Microbiol. 1963; 33:231-42. DOI: 10.1099/00221287-33-2-231. View

13.

Tweats D, Thompson M, Pinney R, Smith J . R factor-mediated resistance to ultraviolet light in strains of Escherichia coli deficient in known repair functions. J Gen Microbiol. 1976; 93(1):103-10. DOI: 10.1099/00221287-93-1-103. View

14.

Babudri N, Monti-Bragadin C . Restoration of mutability in non-mutable Escherichia coli carrying different plasmids. Mol Gen Genet. 1977; 155(3):287-90. DOI: 10.1007/BF00272807. View

15.

Howarth S . Resistance to the bactericidal effect of ultraviolet radiation conferred on Enterobacteria by the colicine factor coli. J Gen Microbiol. 1965; 40(1):43-55. DOI: 10.1099/00221287-40-1-43. View

16.

Kingsbury D, Helinski D . DNA polymerase as a requirement for the maintenance of the bacterial plasmid colicinogenic factor E1. Biochem Biophys Res Commun. 1970; 41(6):1538-44. DOI: 10.1016/0006-291x(70)90562-0. View

17.

Helinski D, Herschman H . Effect of Rec mutations on the activity of colicinogenic factors. J Bacteriol. 1967; 94(3):700-6. PMC: 251941. DOI: 10.1128/jb.94.3.700-706.1967. View

18.

Siccardi A . Effect of R factors and other plasmids on ultraviolet susceptibility and host cell reactivation property of Escherichia coli. J Bacteriol. 1969; 100(1):337-46. PMC: 315397. DOI: 10.1128/jb.100.1.337-346.1969. View

19.

MacPhee D . Effect of rec mutations on the ultraviolet protecting and mutation-enhancing properties of the plasmid R-Utrecht in Salmonella typhimurium. Mutat Res. 1973; 19(3):357-9. DOI: 10.1016/0027-5107(73)90237-6. View

20.

DE LUCIA P, Cairns J . Isolation of an E. coli strain with a mutation affecting DNA polymerase. Nature. 1969; 224(5225):1164-6. DOI: 10.1038/2241164a0. View