Fibronectin Localization in the Rat Glomerulus

Overview

Journal J Cell Biol

Specialty Cell Biology

Date 1980 Dec 1

PMID 7007395

Citations 43

Authors

P J Courtoy

Y S Kanwar

R O Hynes

M G Farquhar

Affiliations

Soon will be listed here.

Abstract

Fibronectin (FN) has been localized in the rat glomerulus using indirect immunolabeling. It was demonstrated in frozen sections by immunofluorescence, in sections of fixed kidneys by both peroxidase and ferritin-labeled antibodies, and in isolated glomerular basement membranes (GBM) with ferritin-labeled antibodies. Complementary and convergent results were obtained with these approaches. FN was most abundant in the mesangial matrix where it was especially concentrated at the interface between the endothelial and mesangial cells. In the peripheral capillary loop, FN was also detected in the laminae rarae (interna and externa) of the GBM--i.e., between the endothelial and epithelial cells, respectively, and the GBM. These findings indicate that FN is an important constituent of the glomerulus, and they are compatible with the assumption that, in the glomerulus, as in cultured cells, FN is involved in cell-to-cell (mesangial-mesangial, mesangial-endothelial) and cell-to-substrate (mesangial cell-mesangial matrix, epithelium-GBM, endothelium-GBM) attachment.

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References

GRAHAM Jr R, Karnovsky M . The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique. J Histochem Cytochem. 1966; 14(4):291-302. DOI: 10.1177/14.4.291. View

Linder E, Vaheri A, Ruoslahti E, WARTIOVAARA J . Distribution of fibroblast surface antigen in the developing chick embryo. J Exp Med. 1975; 142(1):41-9. PMC: 2189869. DOI: 10.1084/jem.142.1.41. View

Kuusela P, Ruoslahti E, Engvall E, Vaheri A . Immunological interspecies cross-reactions of fibroblast surface antigen (fibronectin). Immunochemistry. 1976; 13(8):639-42. DOI: 10.1016/0019-2791(76)90203-2. View

Keski-Oja J, Mosher D, Vaheri A . Cross-linking of a major fibroblast surface-associated glycoprotein (fibronectin) catalyzed by blood coagulation factor XIII. Cell. 1976; 9(1):29-35. DOI: 10.1016/0092-8674(76)90049-0. View

Langer K, Paul N, Thoenes W . The ultrastructural basis of permeability to proteins in normal and nephrotic glomeruli. Electron microscopic and micropuncture studies using ferritin as a tracer. Contrib Nephrol. 1975; 1:92-5. View

Chen L, Maitland N, Gallimore P, McDougall J . Detection of the large external transformation-sensitive protein on some epithelial cells. Exp Cell Res. 1977; 106(1):39-46. DOI: 10.1016/0014-4827(77)90238-5. View

Mautner V, Hynes R . Surface distribution of LETS protein in relation to the cytoskeleton of normal and transformed cells. J Cell Biol. 1977; 75(3):743-68. PMC: 2111579. DOI: 10.1083/jcb.75.3.743. View

Furcht L, Mosher D, Wendelschafer-Crabb G . Immunocytochemical localization of fibronectin (LETS proteins) on the surface of L6 myoblasts: light and electron microscopic studies. Cell. 1978; 13(2):263-71. DOI: 10.1016/0092-8674(78)90195-2. View

Hedman K, Vaheri A, WARTIOVAARA J . External fibronectin of cultured human fibroblasts is predominantly a matrix protein. J Cell Biol. 1978; 76(3):748-60. PMC: 2109997. DOI: 10.1083/jcb.76.3.748. View

10.

Stenman S, Vaheri A . Distribution of a major connective tissue protein, fibronectin, in normal human tissues. J Exp Med. 1978; 147(4):1054-64. PMC: 2184253. DOI: 10.1084/jem.147.4.1054. View

11.

Macarak E, Kirby E, Kirk T, Kefalides N . Synthesis of cold-insoluble globulin by cultured calf endothelial cells. Proc Natl Acad Sci U S A. 1978; 75(6):2621-5. PMC: 392614. DOI: 10.1073/pnas.75.6.2621. View

12.

Chen L, Murray A, Segal R, Bushnell A, Walsh M . Studies on intercellular LETS glycoprotein matrices. Cell. 1978; 14(2):377-91. DOI: 10.1016/0092-8674(78)90123-x. View

13.

Engvall E, Ruoslahti E, Miller E . Affinity of fibronectin to collagens of different genetic types and to fibrinogen. J Exp Med. 1978; 147(6):1584-95. PMC: 2184308. DOI: 10.1084/jem.147.6.1584. View

14.

Birdwell C, GOSPODAROWICZ D, Nicolson G . Identification, localization, and role of fibronectin in cultured bovine endothelial cells. Proc Natl Acad Sci U S A. 1978; 75(7):3273-7. PMC: 392757. DOI: 10.1073/pnas.75.7.3273. View

15.

Yamada K, Olden K . Fibronectins--adhesive glycoproteins of cell surface and blood. Nature. 1978; 275(5677):179-84. DOI: 10.1038/275179a0. View

16.

Hynes R, Destree A . Relationships between fibronectin (LETS protein) and actin. Cell. 1978; 15(3):875-86. DOI: 10.1016/0092-8674(78)90272-6. View

17.

Pettersson E, Colvin R . Cold-insoluble globulin (fibronectin, LETS protein) in normal and diseased human glomeruli: papain-sensitive attachment to normal glomeruli and deposition in crescents. Clin Immunol Immunopathol. 1978; 11(4):425-36. DOI: 10.1016/0090-1229(78)90170-8. View

18.

Culp L, Rollins B, Buniel J, Hitri S . Two functionally distinct pools of glycosaminoglycan in the substrate adhesion site of murine cells. J Cell Biol. 1978; 79(3):788-801. PMC: 2110261. DOI: 10.1083/jcb.79.3.788. View

19.

Singer I . The fibronexus: a transmembrane association of fibronectin-containing fibers and bundles of 5 nm microfilaments in hamster and human fibroblasts. Cell. 1979; 16(3):675-85. DOI: 10.1016/0092-8674(79)90040-0. View

20.

PERKINS M, Ji T, Hynes R . Cross-linking of fibronectin to sulfated proteoglycans at the cell surface. Cell. 1979; 16(4):941-52. DOI: 10.1016/0092-8674(79)90109-0. View