Molecular Cloning of CDNA Sequences Coding for the Major Alpha- and Beta-globin Polypeptides of Adult Xenopus Laevis

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1980 Jun 25

PMID 7001356

Citations 18

Authors

R M Kay

R Harris

R K Patient

J G Williams

Affiliations

Soon will be listed here.

Abstract

This report describes the synthesis and cloning of almost complete DNA copies of the mRNAs encoding the major alpha-globin and major beta-globin of X. laevis. Double-stranded globin cDNA was inserted into the PstI site of the plasmid pBR322 and two cloned recombinants (designated pXG6C1 and pXG8D2) were selected. These were shown to contain almost complete copies of X. laevis globin mRNA. Restriction enzyme maps were determined for each cDNA sequence using the established method of partial digestion of end labelled DNA. However, this procedure was modified such that isolation of individual DNA fragments was no longer required. Each plasmid was shown, by both hybrid arrested translation and filter selection of complementary RNA, to contain a sequence coding for one or other of the two major globin polypeptides. Sufficient DNA sequence information has been determined from each cDNA clone to demonstrate that pXG8D2 contains a beta-globin sequence and pXG6C1 contains an alpha-globin sequence.

Citing Articles

Developmental pattern and molecular identification of globin chains in Xenopus laevis.

Sandmeier E, Gygi D, Wyler T, Nyffenegger U, Weber R Rouxs Arch Dev Biol. 2017; 197(7):406-412.

PMID: 28305747 DOI: 10.1007/BF00398991.

Whole-genome duplication and the functional diversification of teleost fish hemoglobins.

Opazo J, Butts G, Nery M, Storz J, Hoffmann F Mol Biol Evol. 2012; 30(1):140-53.

PMID: 22949522 PMC: 3525417. DOI: 10.1093/molbev/mss212.

Lineage-specific patterns of functional diversification in the alpha- and beta-globin gene families of tetrapod vertebrates.

Hoffmann F, Storz J, Gorr T, Opazo J Mol Biol Evol. 2010; 27(5):1126-38.

PMID: 20047955 PMC: 2877528. DOI: 10.1093/molbev/msp325.

Platypus globin genes and flanking loci suggest a new insertional model for beta-globin evolution in birds and mammals.

Patel V, Cooper S, Deakin J, Fulton B, Graves T, Warren W BMC Biol. 2008; 6:34.

PMID: 18657265 PMC: 2529266. DOI: 10.1186/1741-7007-6-34.

The nucleotide sequence of the major beta-globin mRNA from Xenopus laevis.

G Williams J, Kay R, Patient R Nucleic Acids Res. 1980; 8(18):4247-58.

PMID: 7433108 PMC: 324232. DOI: 10.1093/nar/8.18.4247.

References

Clewell D, Helinski D . Properties of a supercoiled deoxyribonucleic acid-protein relaxation complex and strand specificity of the relaxation event. Biochemistry. 1970; 9(22):4428-40. DOI: 10.1021/bi00824a026. View

Perutz M . Stereochemistry of cooperative effects in haemoglobin. Nature. 1970; 228(5273):726-39. DOI: 10.1038/228726a0. View

Maclean N, Jurd R . The control of haemoglobin synthesis. Biol Rev Camb Philos Soc. 1972; 47(3):393-437. DOI: 10.1111/j.1469-185x.1972.tb00978.x. View

Efstratiadis A, Maniatis T, Kafatos F, Jeffrey A, Vournakis J . Full length and discrete partial reverse transcripts of globin and chorion mRNAs. Cell. 1975; 4(4):367-78. DOI: 10.1016/0092-8674(75)90157-9. View

Southern E . Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975; 98(3):503-17. DOI: 10.1016/s0022-2836(75)80083-0. View

Grunstein M, Hogness D . Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene. Proc Natl Acad Sci U S A. 1975; 72(10):3961-5. PMC: 433117. DOI: 10.1073/pnas.72.10.3961. View

Thomas N, Maclean N . The erythroid cells of anaemic Xenopus laevis. I. Studies on cellular morphology and protein and nucleic acid synthesis during differentiation. J Cell Sci. 1975; 19(3):509-20. DOI: 10.1242/jcs.19.3.509. View

Roychoudhury R, Jay E, Wu R . Terminal labeling and addition of homopolymer tracts to duplex DNA fragments by terminal deoxynucleotidyl transferase. Nucleic Acids Res. 1976; 3(1):101-16. PMC: 342881. DOI: 10.1093/nar/3.1.101. View

Monahan J, McReynolds L, OMalley B . The ovalbumin gene. In vitro enzymatic synthesis and characterization. J Biol Chem. 1976; 251(23):7355-62. View

10.

McDonell M, Simon M, Studier F . Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels. J Mol Biol. 1977; 110(1):119-46. DOI: 10.1016/s0022-2836(77)80102-2. View

11.

Maxam A, Gilbert W . A new method for sequencing DNA. Proc Natl Acad Sci U S A. 1977; 74(2):560-4. PMC: 392330. DOI: 10.1073/pnas.74.2.560. View

12.

Berkner K, Folk W . Polynucleotide kinase exchange reaction: quantitave assay for restriction endonuclease-generated 5'-phosphoroyl termini in DNA. J Biol Chem. 1977; 252(10):3176-84. View

13.

Rigby P, Dieckmann M, Rhodes C, Berg P . Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977; 113(1):237-51. DOI: 10.1016/0022-2836(77)90052-3. View

14.

Berk A, Sharp P . Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids. Cell. 1977; 12(3):721-32. DOI: 10.1016/0092-8674(77)90272-0. View

15.

Battaglia P, Melli M . Isolation of globin messenger RNA of Xenopus laevis. Dev Biol. 1977; 60(2):337-50. DOI: 10.1016/0012-1606(77)90132-4. View

16.

Humphries P, Old R, Coggins L, McShane T, Watson C, Paul J . Recombinant plasmids containing Xenopus laevis globin structural genes derived from complementary DNA. Nucleic Acids Res. 1978; 5(3):905-24. PMC: 342032. DOI: 10.1093/nar/5.3.905. View

17.

Smith D, Searle P, G Williams J . Characterisation of bacterial clones containing DNA sequences derived from Xenopus laevis vitellogenin mRNA. Nucleic Acids Res. 1979; 6(2):487-506. PMC: 327709. DOI: 10.1093/nar/6.2.487. View

18.

G Williams J, Lloyd M . Changes in the abundance of polyadenylated RNA during slime mould development measured using cloned molecular hybridization probes. J Mol Biol. 1979; 129(1):19-35. DOI: 10.1016/0022-2836(79)90056-1. View

19.

Hentschel C, Kay R, G Williams J . Analysis of Xenopus laevis globins during development and erythroid cell maturation and the construction of recombinant plasmids containing sequences derived from adult globin mRNA. Dev Biol. 1979; 72(2):350-63. DOI: 10.1016/0012-1606(79)90124-6. View