Insulin Binding and Insulin Action in Rat Fat Cells After Adrenalectomy

Overview

Journal Diabetologia

Specialty Endocrinology

Date 1980 Oct 1

PMID 7000600

Authors

H Haring

C Calle

A Bug

R Renner

K D. Hepp

W Kemmler

Affiliations

Soon will be listed here.

Abstract

Insulin binding and the effect of insulin on the transport of 3-O-methylglucose, lipogenesis from glucose, glucose oxidation and lipolysis was studied in fat cells of adrenalectomised rats and of a control group of sham-operated rats. The serum insulin level of the adrenalectomised rats (0.7 ng/ml) was lower than that of the controls (1.6 ng/ml). In adrenalectomised rats as compared to sham-operated rats the insulin concentrations causing half-maximal effect were reduced by 50% in lipogenesis and antilipolysis and by 30% in glucose transport. The increase in sensitivity to submaximal insulin concentrations was not observed in glucose oxidation. The maximal responsiveness was unchanged in all test systems. The increase in sensitivity in three of the four studied insulin effects may be related to the 37% increase in the binding capacity of fat cells from adrenalectomised compared with sham-operated rats. The unchanged sensitivity with respect to glucose oxidation indicates possible post-receptor modulation. When adrenalectomised rats were substituted with either insulin or cortisol serum insulin levels were elevated above normal; however, the changes in the receptor were prevented in the cortisol supplemented rats and only partially in the insulin supplemented rats. The observation suggests, that the insulin receptor is regulated not only by the serum insulin level but also by cortisol.

References

Goldfine I, Kahn C, Neville Jr D, Roth J, GARRISON M, Bates R . Decreased binding of insulin to its receptors in rats with hormone induced insulin resistance. Biochem Biophys Res Commun. 1973; 53(3):852-7. DOI: 10.1016/0006-291x(73)90171-x. View

Smith S, Saggerson E . Decreased sensitivity to insulin in white adipose tissue from adrenalectomised rats. Horm Metab Res. 1977; 9(6):474-80. DOI: 10.1055/s-0028-1093503. View

Kasuga M, Akanuma Y, Iwamoto Y, Kosaka K . Insulin binding and glucose metabolism in adipocytes of streptozotocin-diabetic rats. Am J Physiol. 1978; 235(2):E175-82. DOI: 10.1152/ajpendo.1978.235.2.E175. View

Gavin 3rd J, Roth J, Neville Jr D, De Meyts P, Buell D . Insulin-dependent regulation of insulin receptor concentrations: a direct demonstration in cell culture. Proc Natl Acad Sci U S A. 1974; 71(1):84-8. PMC: 387937. DOI: 10.1073/pnas.71.1.84. View

Olefsky J, Johnson J, Liu F, Jen P, Reaven G . The effects of acute and chronic dexamethasone administration on insulin binding to isolated rat hepatocytes and adipocytes. Metabolism. 1975; 24(4):517-27. DOI: 10.1016/0026-0495(75)90076-1. View

Moody A, Stan M, Stan M, GLIEMANN J . A simple free fat cell bioassay for insulin. Horm Metab Res. 1974; 6(1):12-6. DOI: 10.1055/s-0028-1093895. View

Wieringa T, Krans H . Reduced glucose transport and increased binding of insulin in adipocytes from diabetic and fasted rats. Biochim Biophys Acta. 1978; 538(3):563-70. DOI: 10.1016/0304-4165(78)90417-8. View

Jeanrenaud B, Renold A . Studies on rat adipose tissue in vitro. 7. Effects of adrenal cortical hormones. J Biol Chem. 1960; 235:2217-23. View

Le Marchand Y, Loten E, Assimacopoulos-Jeannet F, Forgue M, Freychet P, Jeanrenaud B . Effect of fasting and streptozotocin in the obese-hyperglycemic (ob/ob) mouse. Apparent lack of a direct relationship between insulin binding and insulin effects. Diabetes. 1977; 26(6):582-90. DOI: 10.2337/diab.26.6.582. View

10.

Olefsky J . Decreased insulin binding to adipocytes and circulating monocytes from obese subjects. J Clin Invest. 1976; 57(5):1165-72. PMC: 436769. DOI: 10.1172/JCI108384. View

11.

Kahn C . Insulin resistance, insulin insensitivity, and insulin unresponsiveness: a necessary distinction. Metabolism. 1978; 27(12 Suppl 2):1893-902. DOI: 10.1016/s0026-0495(78)80007-9. View

12.

Di Girolamo M, Mendlinger S, FERTIG J . A simple method to determine fat cell size and number in four mammalian species. Am J Physiol. 1971; 221(3):850-8. DOI: 10.1152/ajplegacy.1971.221.3.850. View

13.

Ip C, TEPPERMAN H, Holohan P, TEPPERMAN J . Insulin binding and insulin response of adipocytes from rats adapted to fat feeding. J Lipid Res. 1976; 17(6):588-99. View

14.

Baxter D, Stanton K, Lazarus N, Keen H . The relation between insulin and adipocyte insulin receptors during treatment of human obesity. Eur J Clin Invest. 1978; 8(6):361-72. DOI: 10.1111/j.1365-2362.1978.tb00866.x. View

15.

Archer J, Gorden P, Gavin 3rd J, Lesniak M, Roth J . Insulin receptors in human circulating lymphocytes: application to the study of insulin resistance in man. J Clin Endocrinol Metab. 1973; 36(4):627-33. DOI: 10.1210/jcem-36-4-627. View

16.

Freychet P, LAUDAT M, Laudat P, Rosselin G, Kahn C, Gorden P . Impairment of insulin binding to the fat cell plasma membrane in the obese hyperglycemic mouse. FEBS Lett. 1972; 25(2):339-342. DOI: 10.1016/0014-5793(72)80519-2. View

17.

Kahn C, Neville Jr D, Gorden P, Freychet P, Roth J . Insulin receptor defect in insulin resistance: studies in the obese-hyperglycimic mouse. Biochem Biophys Res Commun. 1972; 48(1):135-42. DOI: 10.1016/0006-291x(72)90354-3. View

18.

Vinten J, GLIEMANN J, Osterlind K . Exchange of 3-O-methylglucose in isolated fat cells. Concentration dependence and effect of insulin. J Biol Chem. 1976; 251(3):794-800. View

19.

Fernandez B, Saggerson E . Alterations in response of rat white adipocytes to insulin, noradrenaline, corticotropin and glucagon after adrenalectomy. Correction of these changes by adenosine deaminase. Biochem J. 1978; 174(1):111-8. PMC: 1185891. DOI: 10.1042/bj1740111. View

20.

GLIEMANN J, Osterlind K, Vinten J, Gammeltoft S . A procedure for measurement of distribution spaces in isolated fat cells. Biochim Biophys Acta. 1972; 286(1):1-9. DOI: 10.1016/0304-4165(72)90082-7. View