Extrusion of Metabolites from Baker's Yeast During Glucose-induced Acidification

Overview

Journal Folia Microbiol (Praha)

Publisher Springer

Specialty Microbiology

Date 1980 Jan 1

PMID 6998840

Citations 6

Authors

K Sigler

A Knotkova

J Paca

M Wurst

Affiliations

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Abstract

Extrusion of metabolites (glycerol, lactic, malic, and succinic acid) during the medium acidification caused by resting baker's yeast supplied with 200 mM glucose was studied under aerobic and anaerobic conditions and in the absence and presence of 14 mM KCl. The maximum levels of glycerol and of the sum of acids (about 13 and 8 mM, respectively) were attained anaerobically; aerobiosis reduced the levels by 40-50% and the presence of K+ ions by another 10-20%. The time courses of glucose consumption and medium acidification were similar aerobically and anaerobically. The glucose consumption curves exhibited a short plateau about 2 min after glucose addition, caused probably by a rapid osmotic equilibration of glucose across the cell mambrane. Metabolite extrusion indicates that at high glucose concentrations the alcohol dehydrogenase reaction is supplemented by other reactions aiding in the maintenance of a balanced NAD+/NADH ratio in the cells.

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References

Brown C, Johnson B . Influence of the concentration of glucose and galactose on the physiology of Saccharomyces cerevisiae in continuous culture. J Gen Microbiol. 1970; 64(3):279-87. DOI: 10.1099/00221287-64-3-279. View

Sigler K, Knotkova A, Kotyk A . Effect of inhibitors on acid production by baker's yeast. Folia Microbiol (Praha). 1978; 23(6):409-22. DOI: 10.1007/BF02885568. View

Dahlqvist A . METHOD FOR ASSAY OF INTESTINAL DISACCHARIDASES. Anal Biochem. 1964; 7:18-25. DOI: 10.1016/0003-2697(64)90115-0. View

GANCEDO C, Gancedo J, SOLS A . Glycerol metabolism in yeasts. Pathways of utilization and production. Eur J Biochem. 1968; 5(2):165-72. DOI: 10.1111/j.1432-1033.1968.tb00353.x. View

Pena A . Studies on the mechanism of K+ transport in yeast. Arch Biochem Biophys. 1975; 167(2):397-409. DOI: 10.1016/0003-9861(75)90480-4. View

Wurst M, Sigler K, Knotkova A . Gas chromatographic determination of extracellular metabolites produced by baker's yeast during glucose-induced acidification. Folia Microbiol (Praha). 1980; 25(4):306-10. DOI: 10.1007/BF02876610. View

Holzer H, Bernhardt W, Schneider S . [On glycerin formation in baker's yeast]. Biochem Z. 1963; 336:495-509. View

Radler F, Fuck E . [Decomposition of L-malic acid by Saccharomyces cerevisiae during fermentation]. Experientia. 1970; 26(7):731. DOI: 10.1007/BF02232511. View

Ohnishi T, SOTTOCASA G, Ernster L . Current approaches to the mechanism of energy-coupling in the respiratory chain. Studies with yeast mitochondria. Bull Soc Chim Biol (Paris). 1966; 48(11):1189-203. View

10.

Holzer H, HOLZER E, Schultz G . [Relation between growth and aerobic fermentation. I. Experiments with yeast cells]. Biochem Z. 1955; 326(6):385-404. View

11.

CONWAY E, BRADY T . Biological production of acid and alkali; quantitative relations of succinic and carbonic acids to the potassium and hydrogen ion exchange in fermenting yeast. Biochem J. 1950; 47(3):360-9. PMC: 1275220. DOI: 10.1042/bj0470360. View

12.

Aiking H, Sterkenburg A, Tempest D . Influence of specific growth limitation and dilution rate on the phosphorylation efficiency and cytochrome content of mitochondria of Candida utilis NCYC 321. Arch Microbiol. 1977; 113(1-2):65-72. DOI: 10.1007/BF00428582. View

13.

CONWAY E, OMalley E . The nature of the cation exchanges during yeast fermentation, with formation of 0 degrees 02 N-H ion. Biochem J. 2010; 40(1):59-67. View

14.

Rickard P, Hogan C . Effects of glucose on the activity and synthesis of fermentative and respiratory pathway of Saccharomyces sp. Biotechnol Bioeng. 1978; 20(7):1105-10. DOI: 10.1002/bit.260200712. View

15.

Watson T . Effects of sodium chloride on steady-state growth and metabolism of Saccharomyces cerevisiae. J Gen Microbiol. 1970; 64(1):91-9. DOI: 10.1099/00221287-64-1-91. View

16.

Polakis E, Bartley W, Meek G . Changes in the activities of respiratory enzymes during the aerobic growth of yeast on different carbon sources. Biochem J. 1965; 97(1):298-302. PMC: 1264574. DOI: 10.1042/bj0970298. View