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Direct Immunofluorescence of Skin Using Formalin-fixed Paraffin-embedded Sections

Overview
Journal J Clin Pathol
Specialty Pathology
Date 1980 Apr 1
PMID 6995489
Citations 7
Authors
S L Mera
E W Young
J W Bradfield
Affiliations
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Abstract

The technique of direct immunofluorescence has been applied to skin biopsy specimens fixed in formalin and embedded in paraffin wax. The results have been compared with those obtained by using snap-frozen biopsy specimens from the same patients. Trypsinisation of the dewaxed material allowed subsequent detection of immunoglobulins, complement, and fibrinogen. When compared to the fluorescence in the snap-frozen specimens, the staining in the paraffin sections was less bright and there was a higher rate of negatives. Even so, it was possible to establish the diagnosis in most cases of pemphigus, pemphigoid, and lupus erythematosus.

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Direct Immunofluorescence of IgG on Formalin-Fixed Paraffin-Embedded Tissue by Heat-Induced Antigen Retrieval as a Sensitive Method for the Diagnosis of Pemphigus.

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Clinical and histological characterization of oral pemphigus lesions in patients with skin diseases: a cross sectional study from Sudan.

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References
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