Codistribution of Collagen Types IV and AB2 in Basement Membranes and Mesangium of the Kidney. an Immunoferritin Study of Ultrathin Frozen Sections

Overview

Journal J Cell Biol

Specialty Cell Biology

Date 1980 Jun 1

PMID 6993495

Citations 67

Authors

F J Roll

J A Madri

J Albert

H Furthmayr

Affiliations

Soon will be listed here.

Abstract

Affinity-purified rabbit antibodies specific for collagen types I, III, AB2 and for a partially characterized type IV collagen derived from a murine tumor were used to study the distribution of collagens in the normal mouse kidney. Immunofluorescence staining of conventional frozen sections demonstrated that types I and III were present in bundles around large vessels and in fibers surrounding glomeruli and tubules, whereas types IV and AB2 were distributed in a linear fashion along basement membranes of tubules, glomeruli, and Bowman's capsule and in the mesangial stalk. The distribution of types IV nd AB2 was examined at the ultrastructural level by staining of 600- to 800-A thick frozen sections with a three-stage procedure employing specific collagen antibodies, biotinyl sheep antirabbit IgG, and avidin-ferritin conjugates. Labeling by this procedure demonstrated codistribution of types AB2 and the putative type IV in all three basement membranes. In addition, mesangial matrix was shown to contain both of these collagen types. These results support recent biochemical evidence of collagen heterogeneity in basement membranes, and also support the concept of a structural relationship between mesangial matrix and glomerular basement membranes.

Citing Articles

Differential responses of blood-brain barrier associated cells to hypoxia and ischemia: a comparative study.

Engelhardt S, Huang S, Patkar S, Gassmann M, Ogunshola O Fluids Barriers CNS. 2015; 12:4.

PMID: 25879623 PMC: 4429667. DOI: 10.1186/2045-8118-12-4.

Identification of molecular mechanisms used by Finegoldia magna to penetrate and colonize human skin.

Murphy E, Morgelin M, Reinhardt D, Olin A, Bjorck L, Frick I Mol Microbiol. 2014; 94(2):403-17.

PMID: 25164331 PMC: 4241043. DOI: 10.1111/mmi.12773.

A critical role for macrophages in neovessel formation and the development of stenosis in tissue-engineered vascular grafts.

Hibino N, Yi T, Duncan D, Rathore A, Dean E, Naito Y FASEB J. 2011; 25(12):4253-63.

PMID: 21865316 PMC: 3236622. DOI: 10.1096/fj.11-186585.

Antiatherosclerotic and renoprotective effects of ebselen in the diabetic apolipoprotein E/GPx1-double knockout mouse.

Chew P, Yuen D, Stefanovic N, Pete J, Coughlan M, Jandeleit-Dahm K Diabetes. 2010; 59(12):3198-207.

PMID: 20823099 PMC: 2992783. DOI: 10.2337/db10-0195.

Identification and characterization of the endocytic transmembrane glycoprotein Endo180 as a novel collagen receptor.

Wienke D, MacFadyen J, Isacke C Mol Biol Cell. 2003; 14(9):3592-604.

PMID: 12972549 PMC: 196552. DOI: 10.1091/mbc.e02-12-0814.

References

Hauschka S, KONIGSBERG I . The influence of collagen on the development of muscle clones. Proc Natl Acad Sci U S A. 1966; 55(1):119-26. PMC: 285764. DOI: 10.1073/pnas.55.1.119. View

WELLS A, Miller C, NADEL M . Rapid fluorescein and protein assay method for fluorescent-antibody conjugates. Appl Microbiol. 1966; 14(2):271-5. PMC: 546666. DOI: 10.1128/am.14.2.271-275.1966. View

Kefalides N . Isolation and characterization of the collagen from glomerular basement membrane. Biochemistry. 1968; 7(9):3103-12. DOI: 10.1021/bi00849a012. View

Peterkofsky B, Diegelmann R . Use of a mixture of proteinase-free collagenases for the specific assay of radioactive collagen in the presence of other proteins. Biochemistry. 1971; 10(6):988-94. DOI: 10.1021/bi00782a009. View

Kefalides N . Chemical properties of basement membranes. Int Rev Exp Pathol. 1971; 10:1-39. View

Becker J, Wilchek M . Inactivation by avidin of biotin-modified bacteriophage. Biochim Biophys Acta. 1972; 264(1):165-70. DOI: 10.1016/0304-4165(72)90127-4. View

Beil W, Furthmayr H, Timpl R . Chicken antibodies to soluble rat collagen. I. Characterization of the immune response by precipitation and agglutination methods. Immunochemistry. 1972; 9(8):779-88. View

Painter R, Tokuyasu K, Singer S . Immunoferritin localization of intracellular antigens: the use of ultracryotomy to obtain ultrathin sections suitable for direct immunoferritin staining. Proc Natl Acad Sci U S A. 1973; 70(6):1649-53. PMC: 433565. DOI: 10.1073/pnas.70.6.1649. View

Kefalides N . Structure and biosynthesis of basement membranes. Int Rev Connect Tissue Res. 1973; 6:63-104. DOI: 10.1016/b978-0-12-363706-2.50008-8. View

10.

Rowe D, McGoodwin E, Martin G, Sussman M, Grahn D, Faris B . A sex-linked defect in the cross-linking of collagen and elastin associated with the mottled locus in mice. J Exp Med. 1974; 139(1):180-92. PMC: 2139504. DOI: 10.1084/jem.139.1.180. View

11.

Kraehenbuhl J, JAMIESON J . Localization of intracellular antigens by immunoelectron microscopy. Int Rev Exp Pathol. 1974; 13(0):1-53. View

12.

Heitzmann H, Richards F . Use of the avidin-biotin complex for specific staining of biological membranes in electron microscopy. Proc Natl Acad Sci U S A. 1974; 71(9):3537-41. PMC: 433809. DOI: 10.1073/pnas.71.9.3537. View

13.

Daniels J, Chu G . Basement membrane collagen of renal glomerulus. J Biol Chem. 1975; 250(9):3531-7. View

14.

Wick G, Furthmayr H, Timpl R . Purified antibodies to collagen: an immunofluorescence study of their reaction with tissue collagen. Int Arch Allergy Appl Immunol. 1975; 48(5):664-79. DOI: 10.1159/000231354. View

15.

Wick G, Kraft D, Kokschka E, Timpl R . Defined immunofluorescence studies of anticollagen sera. Ann N Y Acad Sci. 1975; 254:614-7. DOI: 10.1111/j.1749-6632.1975.tb29208.x. View

16.

Burgeson R, El Adli F, Kaitila I, Hollister D . Fetal membrane collagens: identification of two new collagen alpha chains. Proc Natl Acad Sci U S A. 1976; 73(8):2579-83. PMC: 430691. DOI: 10.1073/pnas.73.8.2579. View

17.

Hay E, Meier S . Stimulation of corneal differentiation by interaction between cell surface and extracellular matrix. II. Further studies on the nature and site of transfilter "induction". Dev Biol. 1976; 52(1):141-57. DOI: 10.1016/0012-1606(76)90014-2. View

18.

Chung E, Rhodes K, Miller E . Isolation of three collagenous components of probable basement membrane origin from several tissues. Biochem Biophys Res Commun. 1976; 71(4):1167-74. DOI: 10.1016/0006-291x(76)90776-2. View

19.

Tokuyasu K, Singer S . Improved procedures for immunoferritin labeling of ultrathin frozen sections. J Cell Biol. 1976; 71(3):894-906. PMC: 2109786. DOI: 10.1083/jcb.71.3.894. View

20.

Orkin R, Gehron P, McGoodwin E, Martin G, Valentine T, Swarm R . A murine tumor producing a matrix of basement membrane. J Exp Med. 1977; 145(1):204-20. PMC: 2180589. DOI: 10.1084/jem.145.1.204. View