» Articles » PMID: 6987538

Gene Transfer in Intact Animals

Overview
Journal Nature
Specialty Science
Date 1980 Apr 3
PMID 6987538
Citations 24
Authors
Affiliations
Soon will be listed here.
Abstract

Resistance to methotrexate was induced in bone marrow cells of mice by transformation in vitro with DNA from a drug-resistant cell line. Transformed cells were injected in vivo and haematopoietic cells expressing resistance were selected by drug treatment of recipients. Transformed cells had elevated levels of dihydrofolate reductase and demonstrated a proliferative advantage over untransformed cells, indicating successful gene transfer.

Citing Articles

Genetic Disease and Therapy.

Roth T, Marson A Annu Rev Pathol. 2021; 16:145-166.

PMID: 33497260 PMC: 9704033. DOI: 10.1146/annurev-pathmechdis-012419-032626.


A Broad Overview and Review of CRISPR-Cas Technology and Stem Cells.

Waddington S, Privolizzi R, Karda R, ONeill H Curr Stem Cell Rep. 2016; 2:9-20.

PMID: 27398291 PMC: 4913977. DOI: 10.1007/s40778-016-0037-5.


Precision editing of large animal genomes.

Tan W, Carlson D, Walton M, Fahrenkrug S, Hackett P Adv Genet. 2012; 80:37-97.

PMID: 23084873 PMC: 3683964. DOI: 10.1016/B978-0-12-404742-6.00002-8.


Hematopoietic stem cell gene therapy.

Emery D, Nishino T, Murata K, Fragkos M, Stamatoyannopoulos G Int J Hematol. 2002; 75(3):228-36.

PMID: 11999349 DOI: 10.1007/BF02982035.


High-frequency transfer of cloned herpes simplex virus type 1 sequences to mammalian cells by protoplast fusion.

Sandri-Goldin R, Goldin A, Levine M, Glorioso J Mol Cell Biol. 1981; 1(8):743-52.

PMID: 9279387 PMC: 369354. DOI: 10.1128/mcb.1.8.743-752.1981.