Reactivity Differences Among Human T Cells from Blood and Lymphoid Organs, Analysed by Limiting Dilution: Correlation with Specific Gravity and Binding of Peanut Lectin

Overview

Journal Clin Exp Immunol

Publisher Oxford University Press

Specialty Allergy & Immunology

Date 1981 Aug 1

PMID 6976246

Authors

R J van de Griend

R van Doorn

L Stricker

W P Zeijlemaker

D Roos

Affiliations

Soon will be listed here.

Abstract

Human T cells from peripheral blood, cord blood, thymus, spleen and lymph node were analysed for their proliferative response capacity to mitogens, for their specific gravity and size, and for their binding capacity of peanut agglutinin. A close correlation was found between these parameters: thymocytes and T cells from spleen were consistently heavier and smaller, and showed a lower proliferative response capacity, than T cells from blood or lymph node. Similarly, within each cell population, the small, heavy cells were least reactive. The limiting-dilution analysis revealed that heavy T cells from peripheral blood contain a lower number of reactive cells than the light peripheral T lymphocytes. Because heavy T cells from the thymus bound more peanut lectin than did light cells, it is speculated that the differences between T cells of high and low specific gravity might reflect differences in maturation level.

References

Roos D, LOOS J . Changes in the carbohydrate metabolism of mitogenically stimulated human peripheral lymphocytes. I. Stimulation by phytohaemagglutinin. Biochim Biophys Acta. 1970; 222(3):565-82. DOI: 10.1016/0304-4165(70)90182-0. View

van de Griend R, Astaldi A, Vossen J, DOOREN L, Schellekens P, Zwaan F . T lymphocyte characteristics in bone marrow-transplanted patients. I. Changes in biochemical properties that correlate with the immunologic reconstitution. J Immunol. 1981; 126(2):636-40. View

August C, Merler E, Lucas D, Janeway C . The response in vitro of human lymphocytes to phytohemagglutinin and to antigens after fractionation on discontinuous density gradients of albumin. Cell Immunol. 1970; 1(6):603-18. DOI: 10.1016/0008-8749(70)90026-2. View

Konda S, Nakao Y, Smith R . Immunologic properties of mouse thymus cells. Identification of T cell functions within a minor, low-density subpopulation. J Exp Med. 1972; 136(6):1461-77. PMC: 2139338. DOI: 10.1084/jem.136.6.1461. View

Weissman I, Small M, Fathman C, Herzenberg L . Differentiation of thymus cells-1,2. Fed Proc. 1975; 34(2):141-4. View

Shortman K, von Boehmer H, Lipp J, Hopper K . Subpopulations of T-lymphocytes. Physical separation, functional specialisation and differentiation pathways of sub-sets of thymocytes and thymus-dependent peripheral lymphocytes. Transplant Rev. 1975; 25:163-210. View

Roberts I, Whittingham S, Mackay I . Populations of lymphocytes separated from human thymocytes. Clin Exp Immunol. 1975; 19(2):251-9. PMC: 1538097. View

Loos H, van Doorn R, Hoksbergen R, Brutel de la Riviere A, Meerhof L . A method for the recognition and separation of human blood monocytes on density gradients. Blood. 1976; 48(5):731-42. View

Loos H, Kipp B, van Doorn R, Meerhof L . Size distribution, electronic recognition, and counting of human blood monocytes. Blood. 1976; 48(5):743-53. View

10.

PAPIERNIK M, Laroche L, Bach J . Thymocyte subpopulations in young and adult mice. I. Separation by density gradient and steroid treatment. Eur J Immunol. 1977; 7(11):796-9. DOI: 10.1002/eji.1830071110. View

11.

Astaldi G, Astaldi A, Groenewoud M, Wijermans P, Schellekens P, EIJSVOOGEL V . Effect of a human serum thymic factor on hydrocortisone-treated thymocytes. Eur J Immunol. 1977; 7(12):836-40. DOI: 10.1002/eji.1830071203. View

12.

Schut B, Loos H, Roos M, Pinkster T, Zeijlemaker W, Schellekens P . Transformation capacity of human blood lymphocytes in relation to their specific gravity; another heterogeneity of lymphocyte reactivity. Eur J Immunol. 1978; 8(6):386-93. DOI: 10.1002/eji.1830080604. View

13.

London J, Berrih S, Bach J . Peanut agglutinin. I. A new tool for studying T lymphocyte subpopulations. J Immunol. 1978; 121(2):438-43. View

14.

Plum J, Monteyne R, Dhont E, De Smedt M . Lactate dehydrogenase isoenzyme pattern: marker for differentiation of lymphocytes. Scand J Immunol. 1978; 7(6):515-8. DOI: 10.1111/j.1365-3083.1978.tb00486.x. View

15.

van Oers M, Pinkster J, Zeijlemaker W . Quantification of antigen-reactive cells among human T lymphocytes. Eur J Immunol. 1978; 8(7):477-84. DOI: 10.1002/eji.1830080706. View

16.

van Oers M, Pinkster J, Zeijlemaker W . Cooperative effects in mitogen- and antigen-induced responses of human peripheral blood lymphocyte subpopulations. Int Arch Allergy Appl Immunol. 1979; 58(1):53-66. DOI: 10.1159/000232173. View

17.

Reisner Y, Biniaminov M, Rosenthal E, Sharon N, Ramot B . Interaction of peanut agglutinin with normal human lymphocytes and with leukemic cells. Proc Natl Acad Sci U S A. 1979; 76(1):447-51. PMC: 382957. DOI: 10.1073/pnas.76.1.447. View

18.

Maryanski J, MacDonald H, Cerottini J . Limiting dilution analysis of alloantigen-reactive T lymphocytes. IV. High frequency of cytolytic T lymphocyte precursor cells in MLC blasts separated by velocity sedimentation. J Immunol. 1980; 124(1):42-7. View

19.

van de Griend R, Astaldi G, van den Ende A, LOOS J, Roos D . Subpopulations of T lymphocytes from human blood differing in density and stage of maturation. Eur J Immunol. 1980; 10(1):70-3. DOI: 10.1002/eji.1830100114. View

20.

Noorloos A, Van Beek A, Melief C . Cryopreservation of cells for immunological typing of non-Hodgkin's lymphomas. Cancer Res. 1980; 40(8 Pt 1):2890-4. View