Control of B-cell Maturation in Mice. I. Increased B-cell Maturation in Vitro by Bone Marrow Protected During Whole Body Irradiation

Overview

Journal Immunology

Specialty Allergy & Immunology

Date 1981 Feb 1

PMID 6970177

Citations 4

Authors

P P Le Bouteiller

G L Asherson

A J Edwards

Affiliations

Soon will be listed here.

Abstract

The possibility of a homeostatic control on the production of B cells was studied in CBA mice following whole body irradiation (750 rads). Bone marrow cells from femurs shielded from irradiation were taken at 24 h and the number of surface immunoglobulin positive cells assessed with a fluorescence-activated cell sorter after 24 h in vitro. The cells from the irradiated shielded mice showed greater absolute number of 'bright' B cells with a high density of surface immunoglobulin (mean increase 60%--100%) than cells from control unirradiated mice. These bright B cells did not incorporate (3H) thymidine in vitro and treatment with hydroxyurea (an inhibitor of DNA synthesis) did not prevent their increase. It was concluded that the increased number of bright B cells in vitro arose from augmented maturation or differentiation and not from a proliferative process.

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References

Yang W, Miller S, OSMOND D . Maturation of bone marrow lymphocytes. II. Development of Fc and complement receptors and surface immunoglobulin studied by rosetting and radioautography. J Exp Med. 1978; 148(5):1251-70. PMC: 2185056. DOI: 10.1084/jem.148.5.1251. View

GIDALI J, LAJTHA L . Regulation of haemopoietic stem cell turnover in partially irradiated mice. Cell Tissue Kinet. 1972; 5(2):147-57. DOI: 10.1111/j.1365-2184.1972.tb01011.x. View

Streefkerk J . Inhibition of erythrocyte pseudoperoxidase activity by treatment with hydrogen peroxide following methanol. J Histochem Cytochem. 1972; 20(10):829-31. DOI: 10.1177/20.10.829. View

GRAHAM Jr R, Karnovsky M . The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique. J Histochem Cytochem. 1966; 14(4):291-302. DOI: 10.1177/14.4.291. View

Brahim F, OSMOND D . Migration of bone marrow lymphocytes demonstrated by selective bone marrow labeling with thymidine-H3. Anat Rec. 1970; 168(2):139-59. DOI: 10.1002/ar.1091680202. View

HULSE E . Lymphocytic recovery after irradiation and its relation to other aspects of haemopoiesis. Br J Haematol. 1963; 9:376-84. DOI: 10.1111/j.1365-2141.1963.tb06562.x. View

UNANUE E, Grey H, Rabellino E, Campbell P, Schmidtke J . Immunoglobulins on the surface of lymphocytes. II. The bone marrow as the main source of lymphocytes with detectable surface-bound immunoglobulin. J Exp Med. 1971; 133(6):1188-98. PMC: 2138936. DOI: 10.1084/jem.133.6.1188. View

OSMOND D, Nossal G . Differentiation of lymphocytes in mouse bone marrow. II. Kinetics of maturation and renewal of antiglobulin-binding cells studied by double labeling. Cell Immunol. 1974; 13(1):132-45. DOI: 10.1016/0008-8749(74)90233-0. View

Nossal G, Cunningham A, Mitchell G, Miller J . Cell to cell interaction in the immune response. 3. Chromosomal marker analysis of single antibody-forming cells in reconstituted, irradiated, or thymectomized mice. J Exp Med. 1968; 128(4):839-53. PMC: 2138549. DOI: 10.1084/jem.128.4.839. View

10.

Ryser J, Vassalli P . Mouse bone marrow lymphocytes and their differentiation. J Immunol. 1974; 113(3):719-28. View

11.

Harris P . Quantitative examination of bone marrow in guinea-pigs after gamma irradiation; a preliminary note. Br Med J. 1956; 2(5000):1032-5. PMC: 2035669. DOI: 10.1136/bmj.2.5000.1032. View

12.

Sinclair W . Hydroxyurea: effects on Chinese hamster cells grown in culture. Cancer Res. 1967; 27(2):297-308. View

13.

Fuchs B, Khaitov R, Petrov R, Ataullakhanov R, Sidorovich I, Vanko L . Bone marrow suppressor B cells in vitro. Immunology. 1978; 35(6):997-1003. PMC: 1457438. View

14.

Lala P, Johnson G, Battye F, Nossal G . Maturation of B lymphocytes. I. Concurrent appearance of increasing Ig, Ia, and mitogen responsiveness. J Immunol. 1979; 122(1):334-41. View

15.

Anderson R, Olson G, Autry J, Howarth J, TROUP G, Bartels P . Radiosensitivity of T and B lymphocytes. IV. Effect of whole body irradiation upon various lymphoid tissues and numbers of recirculating lymphocytes. J Immunol. 1977; 118(4):1191-200. View

16.

Avrameas S, Ternynck T . Peroxidase labelled antibody and Fab conjugates with enhanced intracellular penetration. Immunochemistry. 1971; 8(12):1175-9. DOI: 10.1016/0019-2791(71)90395-8. View

17.

PreudHomme J, Flandrin G . Identification by peroxidase staining of monocytes in surface immunofluorescence tests. J Immunol. 1974; 113(5):1650-3. View

18.

Janossy G, Greaves M . Functional analysis of murine and human B lymphocyte subsets. Transplant Rev. 1975; 24:177-236. DOI: 10.1111/j.1600-065x.1975.tb00169.x. View

19.

Loken M, Herzenber L . Analysis of cell populations with a fluorescence-activated cell sorter. Ann N Y Acad Sci. 1975; 254:163-71. DOI: 10.1111/j.1749-6632.1975.tb29166.x. View

20.

Owen J, Wright D, Habu S, Raff M, Cooper M . Studies on the generation of B lymphocytes in fetal liver and bone marrow. J Immunol. 1977; 118(6):2067-72. View