Interspersion of Repetitive with Repetitive Sequences in an Amphibian, Rana Berlandieri

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1980 Sep 11

PMID 6969394

Citations 1

Authors

D E Graham

K E Schanke

Affiliations

Soon will be listed here.

Abstract

When conventional genome arrangement analyses performed on R. berlandieri DNA at a normal (60 degrees C) and a high (75 degrees C) reassociation temperature were compared, an additional interspersion pattern was detected which indicates that different classes of repetitive sequences are closely interspersed with each other. Our results further suggest that the genomic abundance of purified (or cloned) repetitive sequences can be accurately determined by solution hybridization with genomic DNA only when the reassociation is performed at a relatively high temperature (Tm - 10 degrees C).

Citing Articles

Structural characteristics of genome organization in amphibians: differential staining of chromosomes and DNA structure.

Birstein V J Mol Evol. 1982; 18(2):73-91.

PMID: 7047753 DOI: 10.1007/BF01810826.

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