Assignment of Two Mitochondrially Synthesized Polypeptides to Human Mitochondrial DNA and Their Use in the Study of Intracellular Mitochondrial Interaction

Overview

Journal Mol Cell Biol

Specialty Cell Biology

Date 1982 Jan 1

PMID 6955589

Citations 25

Authors

N A Oliver

D C Wallace

Affiliations

Soon will be listed here.

Abstract

Two mitochondrially synthesized marker polypeptides, MV-1 and MV-2, were found in human HeLa and HT1080 cells. These were assigned to the mitochondrial DNA in HeLa-HT1080 cybrids and hybrids by demonstrating their linkage to cytoplasmic genetic markers. These markers include mitochondrial DNA restriction site polymorphisms and resistance to chloramphenicol, an inhibitor of mitochondrial protein synthesis. In the absence of chloramphenicol, the expression of MV-1 and MV-2 in cybrids and hybrids was found to be directly proportional to the ratio of the parental mitochondrial DNAs. In the presence of chloramphenicol, the marker polypeptide linked to the chloramphenicol-sensitive mitochondrial DNA continued to be expressed. This demonstrated that resistant and sensitive mitochondrial DNAs can cooperate within a cell for gene expression and that the CAP-resistant allele was dominant or codominant to sensitive. Such cooperation suggests that mitochondrial DNAs can be exchanged between mitochondria.

Citing Articles

Off-Target Effects of Drugs that Disrupt Human Mitochondrial DNA Maintenance.

Young M Front Mol Biosci. 2017; 4:74.

PMID: 29214156 PMC: 5702650. DOI: 10.3389/fmolb.2017.00074.

Progressive increase in mtDNA 3243A>G heteroplasmy causes abrupt transcriptional reprogramming.

Picard M, Zhang J, Hancock S, Derbeneva O, Golhar R, Golik P Proc Natl Acad Sci U S A. 2014; 111(38):E4033-42.

PMID: 25192935 PMC: 4183335. DOI: 10.1073/pnas.1414028111.

Mitochondrial DNA genetics and the heteroplasmy conundrum in evolution and disease.

Wallace D, Chalkia D Cold Spring Harb Perspect Biol. 2013; 5(11):a021220.

PMID: 24186072 PMC: 3809581. DOI: 10.1101/cshperspect.a021220.

Heteroplasmy of mouse mtDNA is genetically unstable and results in altered behavior and cognition.

Sharpley M, Marciniak C, Eckel-Mahan K, McManus M, Crimi M, Waymire K Cell. 2012; 151(2):333-343.

PMID: 23063123 PMC: 4175720. DOI: 10.1016/j.cell.2012.09.004.

Mitochondria and cancer.

Wallace D Nat Rev Cancer. 2012; 12(10):685-98.

PMID: 23001348 PMC: 4371788. DOI: 10.1038/nrc3365.

References

de Jong L, Holtrop M, Kroon A . The biogenesis of rat-liver mitochondrial ATPase. Evidence that the N, N'-dicyclohexyl carbodiimide-binding protein is synthesized outside the mitochondria. Biochim Biophys Acta. 1980; 606(2):331-7. DOI: 10.1016/0005-2787(80)90042-8. View

Wiseman A, Attardi G . Cytoplasmically inherited mutations of a human cell line resulting in deficient mitochondrial protein synthesis. Somatic Cell Genet. 1979; 5(2):241-62. DOI: 10.1007/BF01539164. View

Apirion D . Three genes that affect Escherichia coli ribosomes. J Mol Biol. 1967; 30(2):255-75. View

Chen T . In situ detection of mycoplasma contamination in cell cultures by fluorescent Hoechst 33258 stain. Exp Cell Res. 1977; 104(2):255-62. DOI: 10.1016/0014-4827(77)90089-1. View

Wallace D, Bunn C, Eisenstadt J . Cytoplasmic transfer of chloramphenicol resistance in human tissue culture cells. J Cell Biol. 1975; 67(1):174-88. PMC: 2109574. DOI: 10.1083/jcb.67.1.174. View

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

Nass M . Mitochondrial DNA. I. Intramitochondrial distribution and structural relations of single- and double-length circular DNA. J Mol Biol. 1969; 42(3):521-8. DOI: 10.1016/0022-2836(69)90240-x. View

Linnane A, Saunders G, Gingold E, Lukins H . The biogenesis of mitochondria. V. Cytoplasmic inheritance of erythromycin resistance in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1968; 59(3):903-10. PMC: 224776. DOI: 10.1073/pnas.59.3.903. View

Rasheed S, Nelson-Rees W, Toth E, ARNSTEIN P, Gardner M . Characterization of a newly derived human sarcoma cell line (HT-1080). Cancer. 1974; 33(4):1027-33. DOI: 10.1002/1097-0142(197404)33:4<1027::aid-cncr2820330419>3.0.co;2-z. View

10.

de Jong L, Holtrop M, Kroon A . The biogenesis of rat mitochondrial ATPase. Two subunits are synthesized inside the mitochondria. Biochim Biophys Acta. 1980; 608(1):32-8. DOI: 10.1016/0005-2787(80)90130-6. View

11.

Kit S, DUBBS D, FREARSON P . HeLa cells resistant to bromodeoxyuridine and deficient in thymidine kinase activity. Int J Cancer. 1966; 1(1):19-30. DOI: 10.1002/ijc.2910010105. View

12.

Spolsky C, Eisenstadt J . Chloramphenicol-resistant mutants of human HeLa cells. FEBS Lett. 1972; 25(2):319-324. DOI: 10.1016/0014-5793(72)80514-3. View

13.

Birky Jr C . On the origin of mitochondrial mutants: evidence for intracellular selection of mitochondria in the origin of antibiotic-resistant cells in yeast. Genetics. 1973; 74(3):421-32. PMC: 1212959. DOI: 10.1093/genetics/74.3.421. View

14.

Costantino P, Attardi G . Identification of discrete electrophoretic components among the products of mitochondrial protein synthesis in HeLa cells. J Mol Biol. 1975; 96(2):291-306. DOI: 10.1016/0022-2836(75)90349-6. View

15.

Dujon B, SLONIMSKI P, Weill L . Mitochondrial genetics IX: A model for recombination and segregation of mitochondrial genomes in saccharomyces cerevisiae. Genetics. 1974; 78(1):415-37. PMC: 1213202. DOI: 10.1093/genetics/78.1.415. View

16.

Smith C, Jordan J, Vinograd J . In vivo effects of intercalating drugs on the superhelix density of mitochondrial DNA isolated from human and mouse cells in culture. J Mol Biol. 1971; 59(2):255-72. DOI: 10.1016/0022-2836(71)90050-7. View

17.

Battey J, Clayton D . The transcription map of human mitochondrial DNA implicates transfer RNA excision as a major processing event. J Biol Chem. 1980; 255(23):11599-606. View

18.

Nomura M, ENGBAEK F . Expression of ribosomal protein genes as analyzed by bacteriophage Mu-induced mutations. Proc Natl Acad Sci U S A. 1972; 69(6):1526-30. PMC: 426741. DOI: 10.1073/pnas.69.6.1526. View

19.

Eperon I, Anderson S, NIERLICH D . Distinctive sequence of human mitochondrial ribosomal RNA genes. Nature. 1980; 286(5772):460-7. DOI: 10.1038/286460a0. View

20.

Freeman K . Action of the N-trifluoroacetyl analogue of D-chloramphenicol. Antimicrob Agents Chemother. 1977; 11(3):563-5. PMC: 352021. DOI: 10.1128/AAC.11.3.563. View