Construction of a Genetic Map for Caulobacter Crescentus

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1982 Mar 1

PMID 6949897

Citations 29

Authors

J T Barrett

C S Rhodes

D M Ferber

B Jenkins

S A Kuhl

B Ely

Affiliations

Soon will be listed here.

Abstract

RP4-mediated conjugation has been used to transfer large fragments of chromosomal material in Caulobacter crescentus. In this system, conjugation proceeds from multiple origins, and haploid recombinants are recovered at frequencies of 10(-6) and 10(-7) per donor cell. The data from five-factor crosses were subjected to computer-assisted crossover analyses as a rapid method to determine marker order. Using this information and data from additional two- and three-factor crosses mediated by RP4 or the generalized transducing bacteriophage phi Cr30, we constructed the first genetic map for C. crescentus.

Citing Articles

Synchronized Swarmers and Sticky Stalks: Caulobacter crescentus as a Model for Bacterial Cell Biology.

Barrows J, Goley E J Bacteriol. 2023; 205(2):e0038422.

PMID: 36715542 PMC: 9945503. DOI: 10.1128/jb.00384-22.

Recombination and gene loss occur simultaneously during bacterial horizontal gene transfer.

Ely B PLoS One. 2020; 15(1):e0227987.

PMID: 31990919 PMC: 6986707. DOI: 10.1371/journal.pone.0227987.

The Caulobacter crescentus transducing phage Cr30 is a unique member of the T4-like family of myophages.

Ely B, Gibbs W, Diez S, Ash K Curr Microbiol. 2015; 70(6):854-8.

PMID: 25773204 PMC: 4418941. DOI: 10.1007/s00284-015-0799-5.

Isolation of a Caulobacter gene cluster specifying flagellum production by using nonmotile Tn5 insertion mutants.

Purucker M, Bryan R, Amemiya K, Ely B, Shapiro L Proc Natl Acad Sci U S A. 1982; 79(22):6797-801.

PMID: 16593248 PMC: 347220. DOI: 10.1073/pnas.79.22.6797.

Isolation and Characterization of Marine Caulobacters and Assessment of Their Potential for Genetic Experimentation.

Anast N, Smit J Appl Environ Microbiol. 1988; 54(3):809-817.

PMID: 16347590 PMC: 202545. DOI: 10.1128/aem.54.3.809-817.1988.

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