Purification of Thymus MRNA Coding for a 16,000-dalton Polypeptide Containing the Thymosin Alpha 1 Sequence

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1981 Jan 1

PMID 6941243

Citations 3

Authors

M Freire

E Hannappel

M Rey

J M Freire

H Kido

B L HORECKER

Affiliations

Soon will be listed here.

Abstract

A mRNA fraction purified by preparative polyacrylamide disc gel electrophoresis from calf thymus polysomes codes for a polypeptide(s) having a mass of 16,000-17,000 daltons. This polypeptide contains amino acid sequences corresponding to residues 11-18 and 19-25 of thymosin alpha 1. The yield of the octapeptide indicates that the 16,000-dalton peptide is the major product formed in the cell-free synthesis system containing the purified mRNA.

Citing Articles

Thymosin β4 is essential for thrombus formation by controlling the G-actin/F-actin equilibrium in platelets.

Scheller I, Beck S, Gob V, Gross C, Neagoe R, Aurbach K Haematologica. 2021; 107(12):2846-2858.

PMID: 34348450 PMC: 9713564. DOI: 10.3324/haematol.2021.278537.

Involution patterns of the human thymus. I Size of the cortical area as a function of age.

Tosi P, Kraft R, Luzi P, Cintorino M, FANKHAUSER G, Hess M Clin Exp Immunol. 1982; 47(2):497-504.

PMID: 7075032 PMC: 1536525.

Thymosin alpha 11: a peptide related to thymosin alpha 1 isolated from calf thymosin fraction 5.

Caldarella J, Goodall G, Felix A, Heimer E, SALVIN S, HORECKER B Proc Natl Acad Sci U S A. 1983; 80(24):7424-7.

PMID: 6369316 PMC: 389963. DOI: 10.1073/pnas.80.24.7424.

References

Loening U . The fractionation of high-molecular-weight ribonucleic acid by polyacrylamide-gel electrophoresis. Biochem J. 1967; 102(1):251-7. PMC: 1270235. DOI: 10.1042/bj1020251. View

Palmiter R . Magnesium precipitation of ribonucleoprotein complexes. Expedient techniques for the isolation of undergraded polysomes and messenger ribonucleic acid. Biochemistry. 1974; 13(17):3606-15. DOI: 10.1021/bi00714a032. View

Pinder J, Staynov D, Gratzer W . Electrophoresis of RNA in formamide. Biochemistry. 1974; 13(26):5373-8. DOI: 10.1021/bi00723a019. View

Bohlen P, Stein S, Stone J, UDENFRIEND S . Automatic Monitoring of primary amines in preparative column effluents with fluorescamine. Anal Biochem. 1975; 67(2):438-45. DOI: 10.1016/0003-2697(75)90316-4. View

Rubinstein M . Preparative high-performance liquid partition chromatography of proteins. Anal Biochem. 1979; 98(1):1-7. DOI: 10.1016/0003-2697(79)90697-3. View