Two Relative Efficiencies of Polymorphic Enzymes for Characterizing Cell Lines, Detecting Contaminations, and Monitoring Transplants

Overview

Journal In Vitro

Specialties Anatomy & Histology
Cell Biology

Date 1980 Oct 1

PMID 6932388

Citations 1

Authors

W C Wright

Affiliations

Soon will be listed here.

Abstract

A new calculation of the relative efficiency of polymorphic enzyme markers, called the REB, was determined and compared with one of Fisher's determinations of the relative efficiency called REA here. The REA estimates the chance of failing, and 1-REA of succeeding, to show a phenotypic difference between two randomly selected persons or cultured cell lines (Case 1). In this study it was shown that the REA also estimates the chance of detecting a cell line mislabeling or similar mixup (Case 2) and a cell line cross-contamination leading to the complete replacement of an original line by contaminating line (Case 3). The new REB determines the probability of failing, and 1 - REB of succeeding, to detect a contamination of an original line by another line leading to their coexistence, or at least a sufficiently long period of transitional coexistence before one overgrows the other. The REA and REB also apply to determining the efficiency of polymorphic markers in detecting donor and recipient cells in tissue transplants.

Citing Articles

Phenotyping human leukemic T-cell lines: enzyme markers, surface antigens, and cytogenetics.

Martin P, GIBLETT E, Hansen J Immunogenetics. 1982; 15(4):385-98.

PMID: 6978847 DOI: 10.1007/BF00364262.

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