Assignment of Functions to Segments of the DsRNA Genome of the Ustilago Virus

Overview

Journal Mol Gen Genet

Specialties Genetics
Molecular Biology

Date 1980 Apr 1

PMID 6929946

Citations 14

Authors

Y Koltin

R Levine

T Peery

Affiliations

Soon will be listed here.

Abstract

The genome of the 3 Ustilago maydis viruses, known to be associated with the "killer phenomenon", is segmented. The distribution of the viral functions on different segments has been partly resolved. The reported comparative study, of a series of mutants with deleted segments of the genome and of hybrid variants containing segments of the related viruses, provides additional information on the location of various functions and on the interrelations among the different segments. The data indicates that the functions related to the maintenance of the viral information are located on the H segments. Among these segments some function overlap exists. The maintenance of the M and L segments is dependent on the functions located on the H segments. The functions related to the killer phenotype are distributed on the M2 and L1 segments. These functions include the determination of the killer specificity. The H segments play no role in the determination of the killer specificity and the specificity is determined by the M2 segment. The L segment is essential for the expression of the killer phenotype but it is suggested that the information for the toxin resides in the M2 segment.

Citing Articles

The exclusion among the Ustilago viruses - A dsRNA restriction modification system?.

Koltin Y, Levine R, Tamarkin A Curr Genet. 2013; 2(3):185-92.

PMID: 24189908 DOI: 10.1007/BF00435684.

Dual toxin specificities and the exclusion relations among the Ustilago dsRNA viruses.

Wigderson M, Koltin Y Curr Genet. 2013; 5(2):127-36.

PMID: 24186228 DOI: 10.1007/BF00365703.

Yeast killer systems.

Magliani W, Conti S, Gerloni M, Bertolotti D, Polonelli L Clin Microbiol Rev. 1997; 10(3):369-400.

PMID: 9227858 PMC: 172926. DOI: 10.1128/CMR.10.3.369.

Mutants of Ustilago maydis defective in production of one of two polypeptides of KP6 toxin from the preprotoxin.

Tao J, Ginzberg I, Koltin Y, Bruenn J Mol Gen Genet. 1993; 238(1-2):234-40.

PMID: 8479428 DOI: 10.1007/BF00279552.

New developments in fungal virology.

Ghabrial S Adv Virus Res. 1994; 43:303-88.

PMID: 8191957 PMC: 7172821. DOI: 10.1016/s0065-3527(08)60052-4.

References

Bozarth R, Harley E . The electrophoretic mobility of double-stranded RNA in polyacrylamide gels as a function of molecular weight. Biochim Biophys Acta. 1976; 432(3):329-35. DOI: 10.1016/0005-2787(76)90142-8. View

Palfree R, Bussey H . Yeast killer toxin: purification and characterisation of the protein toxin from Saccharomyces cerevisiae. Eur J Biochem. 1979; 93(3):487-93. DOI: 10.1111/j.1432-1033.1979.tb12847.x. View

Franklin R . Purification and properties of the replicative intermediate of the RNA bacteriophage R17. Proc Natl Acad Sci U S A. 1966; 55(6):1504-11. PMC: 224351. DOI: 10.1073/pnas.55.6.1504. View

Koltin Y, Day P . Inheritance of killer phenotypes and double-stranded RNA in Ustilago maydis. Proc Natl Acad Sci U S A. 1976; 73(2):594-8. PMC: 335957. DOI: 10.1073/pnas.73.2.594. View

Wickner R . The killer double-stranded RNA plasmids of yeast. Plasmid. 1979; 2(3):303-22. DOI: 10.1016/0147-619x(79)90015-5. View

Lemke P . Viruses of eucaryotic microorganisms. Annu Rev Microbiol. 1976; 30:105-45. DOI: 10.1146/annurev.mi.30.100176.000541. View

Koltin Y, Day P . Suppression of the killer phenotype in Ustilago maydis. Genetics. 1976; 82(4):629-37. PMC: 1213484. DOI: 10.1093/genetics/82.4.629. View

Levine R, Koltin Y, Kandel J . Nuclease activity associated with the Ustilago maydis virus induced killer proteins. Nucleic Acids Res. 1979; 6(12):3717-31. PMC: 327973. DOI: 10.1093/nar/6.12.3717. View

Puhalla J . Compatibility reactions on solid medium and interstrain inhibition in Ustilago maydis. Genetics. 1968; 60(3):461-74. PMC: 1212055. DOI: 10.1093/genetics/60.3.461. View

10.

Bussey H . Chromosomal mutants of Saccharomyces cerevisiae affecting the cell wall binding site for killer factor. Can J Microbiol. 1978; 24(3):228-37. DOI: 10.1139/m78-041. View

11.

Koltin Y . Virus-like particles in Ustilago maydis: mutants with partial genomes. Genetics. 1977; 86(3):527-34. PMC: 1213692. DOI: 10.1093/genetics/86.3.527. View

12.

Bussey H, Sherman D . Yeast killer factor: ATP leakage and coordinate inhibition of macromolecular synthesis in sensitive cells. Biochim Biophys Acta. 1973; 298(4):868-75. DOI: 10.1016/0005-2736(73)90391-x. View

13.

Koltin Y, Kandel J . Killer Phenomenon in USTILAGO MAYDIS: The Organization of the Viral Genome. Genetics. 1978; 88(2):267-76. PMC: 1213799. DOI: 10.1093/genetics/88.2.267. View

14.

Koltin Y, Mayer I, Steinlauf R . Killer phenomenon in Ustilago maydis: mapping viral functions. Mol Gen Genet. 1978; 166(2):181-6. DOI: 10.1007/BF00285920. View