Article: Chemical and photoelectron spectrometry analysis of the adsorption of phospholipid model membranes and red blood cell membranes on to chrysotile fibres

A study of the interaction of phospholipid model membranes and red blood cell membranes with UICC A chrysotile fibres using chemical analysis and photoelectron spectrometry showed that the interaction agreed with an adsorption of the membranes on to the chrysotile fibres. The photoelectron spectrometry analysis allowed the statement that phospholipid model membranes are adsorbed as bilayer. Chemical analysis showed that for each milligram of chrysotile the amount of phospholipids adsorbed was about 155 microgram and the available surface for phospholipids was about 38 m/g. It was established that entire membranes were adsorbed. A mechanism for the haemolytic capacity of chrysotile is suggested.

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References

1.

Lecuyer H, DERVICHIAN D . Structure of aqueous mixtures of lecithin and cholesterol. J Mol Biol. 1969; 45(1):39-57. DOI: 10.1016/0022-2836(69)90208-3. View

2.

Harington J, Miller K, MACNAB G . Hemolysis by asbestos. Environ Res. 1971; 4(2):95-117. DOI: 10.1016/0013-9351(71)90038-7. View

3.

Jones B, Edwards J, Wagner J . Absorption of serum proteins by inorganic dusts. Br J Ind Med. 1972; 29(3):287-92. PMC: 1009426. DOI: 10.1136/oem.29.3.287. View

4.

Desai R, Hext P, Richards R . The prevention of asbestos-induced hemolysis. Life Sci. 1975; 16(12):1931-8. DOI: 10.1016/0024-3205(75)90303-3. View

5.

Harwood J, Desai R, Hext P, Tetley T, Richards R . Characterization of pulmonary surfactant from ox, rabbit, rat and sheep. Biochem J. 1975; 151(3):707-14. PMC: 1172420. DOI: 10.1042/bj1510707. View

6.

Light W, Wei E . Surface charge and asbestos toxicity. Nature. 1977; 265(5594):537-9. DOI: 10.1038/265537a0. View

7.

Light W, Wei E . Surface charge and hemolytic activity of asbestos. Environ Res. 1977; 13(1):135-45. DOI: 10.1016/0013-9351(77)90012-3. View

8.

Millard M, Bartholomew J . Surface studies of mammalian cells grown in culture by X-ray photoelectron spectroscopy. Anal Chem. 1977; 49(9):1290-6. DOI: 10.1021/ac50017a004. View

9.

Desai R, Richards R . The adsorption of biological macromolecules by mineral dusts. Environ Res. 1978; 16(1-3):449-64. DOI: 10.1016/0013-9351(78)90178-0. View

10.

Jaurand M, Magne L, Bignon J . Inhibition by phospholipids of haemolytic action of asbestos. Br J Ind Med. 1979; 36(2):113-6. PMC: 1008525. DOI: 10.1136/oem.36.2.113. View

11.

Jaurand M, Thomassin J, Magne L, Baillif P . [Adsorption of phospholipids by chrysotile fibers : comparison of data from chemical analysis and photoelectron spectrometry (PSX method)]. C R Seances Acad Sci D. 1979; 288(2):279-82. View

12.

LOWRY O, ROSEBROUGH N, FARR A, RANDALL R . Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; 193(1):265-75. View

Chemical and Photoelectron Spectrometry Analysis of the Adsorption of Phospholipid Model Membranes and Red Blood Cell Membranes on to Chrysotile Fibres