Serum Protein Depletion by Cultured Post-implantation Rat Embryos

Overview

Journal Experientia

Specialty Science

Date 1983 Sep 15

PMID 6884493

Citations 1

Authors

P K Priscott

P G Gough

R D Barnes

Affiliations

Soon will be listed here.

Abstract

Four rat serum proteins were found to be selectively depleted from the culture medium by 10 day rat embryos. These were alpha 2-macroglobulin, transferrin and 2 other proteins which had molecular weights of 132,000 and 214,000. Possibly a 5th protein, alpha 1-acid glycoprotein, was also depleted but its concentration was already low at the start of culture.

Citing Articles

The effect of macromolecular rat serum fractions on conceptuses cultured in human serum: role of transferrin.

Gulamhusein A, Pratten M, Williams C, Beck F J Anat. 1990; 168:113-21.

PMID: 2323987 PMC: 1256894.

References

Healy G, Parker R . Cultivation of mammalian cells in defined media with protein and nonprotein supplements. J Cell Biol. 1966; 30(3):539-53. PMC: 2107029. DOI: 10.1083/jcb.30.3.539. View

Freeman S, Beck F, Lloyd J . The role of the visceral yolk sac in mediating protein utilization by rat embryos cultured in vitro. J Embryol Exp Morphol. 1981; 66:223-34. View

Priscott P . Rat post-implantation embryo culture using heterologous serum. Aust J Exp Biol Med Sci. 1983; 61(Pt 1):47-55. DOI: 10.1038/icb.1983.5. View

Ekblom P, Thesleff I, Miettinen A, SAXEN L . Organogenesis in a defined medium supplemented with transferrin. Cell Differ. 1981; 10(5):281-8. DOI: 10.1016/0045-6039(81)90010-5. View

Cockroft D . Nutrient requirements of rat embryos undergoing organogenesis in vitro. J Reprod Fertil. 1979; 57(2):505-10. DOI: 10.1530/jrf.0.0570505. View

NEW D, Coppola P, Terry S . Culture of explanted rat embryos in rotating tubes. J Reprod Fertil. 1973; 35(1):135-8. DOI: 10.1530/jrf.0.0350135. View

Gauthier F, MOURAY H . Rat alpha2 acute-phase macroglobulin. Isolation and physicochemical properties. Biochem J. 1976; 159(3):661-5. PMC: 1164166. DOI: 10.1042/bj1590661. View

WARDI A, Michos G . Alcian blue staining of glycoproteins in acrylamide disc electrophoresis. Anal Biochem. 1972; 49(2):607-9. DOI: 10.1016/0003-2697(72)90472-1. View

NEW D, Coppola P, Cockroft D . Improved developement of head-fold rat embryos in culture resulting from low oxygen and modifications of the culture serum. J Reprod Fertil. 1976; 48(1):219-22. DOI: 10.1530/jrf.0.0480219. View

10.

GUNBERG D . In vitro development of postimplantation rat embryos cultured on dialyzed rat serum. Teratology. 1976; 14(1):65-9. DOI: 10.1002/tera.1420140109. View

11.

Healy G, Parker R . Selective nucleolar uptake of alpha-1-acid glycoprotein by mammalian cells in tissue culture. Biochim Biophys Acta. 1967; 148(2):556-8. DOI: 10.1016/0304-4165(67)90155-9. View

12.

Priscott P . Commercially available equipment suitable for post-implantation embryo culture. Experientia. 1979; 35(10):1414-5. DOI: 10.1007/BF01964043. View

13.

NEW D . Whole-embryo culture and the study of mammalian embryos during organogenesis. Biol Rev Camb Philos Soc. 1978; 53(1):81-122. DOI: 10.1111/j.1469-185x.1978.tb00993.x. View

14.

KLEIN N, Minghetti P, Jackson S, Vogler M . Serum protein depletion by cultured rat embryos (1). J Exp Zool. 1978; 203(2):313-8. DOI: 10.1002/jez.1402030214. View

15.

Gordon A . The alpha macroglobulins of rat serum. Biochem J. 1976; 159(3):643-50. PMC: 1164164. DOI: 10.1042/bj1590643. View