Platelet Aggregation in Platelet Suspensions from Mongolian Gerbils (Meriones Unguiculatus)

Overview

Journal Can J Comp Med

Specialty Veterinary Medicine

Date 1983 Apr 1

PMID 6883186

Citations 2

Authors

D E Agwu

B J Holub

I B Johnstone

S Crane

Affiliations

Soon will be listed here.

Abstract

Blood obtained from Mongolian gerbils (Meriones unguiculatus) by cardiac puncture was used to develop a method for preparing platelet suspensions suitable for biochemical and aggregometric studies. The aggregation responses of gerbil platelet suspensions to 10 microM adenosine diphosphate and to 0.2 U/mL thrombin were immediate and irreversible. In addition, thrombin produced a short lag period. Bovine acid-soluble collagen (1:800) produced a long lag period coupled with an irreversible aggregation. Indomethacin (10 microM) significantly depressed adenosine diphosphate--induced aggregation but had no effect on thrombin-induced aggregation. Indomethacin significantly prolonged the lag period and reduced the rate of collagen-induced aggregation. Our results strongly indicate that platelet suspensions from the Mongolian gerbil, an animal which is responsive to hypercholesterolemic diets, may be useful in studying dietary lipid factors which influence platelet aggregation.

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