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Electro-optical Measurements on Aqueous Suspension of Purple Membrane from Halobacterium Halobium

Overview
Journal Biophys J
Publisher Cell Press
Specialty Biophysics
Date 1983 Jul 1
PMID 6882862
Citations 15
Authors
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Abstract

The permanent dipole moment, polarizability, and the retinal angle of Halobacterium halobium purple membranes were determined at different pH values. All of the parameters have a maximum between pH 5 and 6. There is a reversal in the direction of the permanent dipole moment near pH 5. The value of permanent dipole moment was determined to be 60 D/protein at pH 6.6, and the value obtained for polarizability was 3 X 10(-28) Fm2/membrane fragment. The retinal angle of all-trans retinal was 0.8 degrees smaller than that of the 13-cis conformation.

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