Structural Elements in Adenovirus Cores. Studies by Means of Freeze-fracturing and Ultrathin Sectioning

Overview

Journal Arch Virol

Specialty Microbiology

Date 1978 Jan 1

PMID 687118

Citations 3

Authors

M V Nermut

Affiliations

Soon will be listed here.

Abstract

Rod-like elements have been observed in adenovirions after freeze-fracturing of purified, semi-purified and intra-cellular adenovirus type 5. Their rod-like shape was clearly demonstrated by means of stereomicrographs. Ultrathin sections prepared in parallel with the freeze-fracture replicas revealed linear structures such as ribbons, rings or rods in up to 30 per cent of the semi-purified or intracellular virus particles. These observations indicate that the main 'structural element' in the adenovirus interior is a linear structure either fragmented into 5 to 6 'rods' or folded 5 to 6 times to fit into the inner space of the virion.

Citing Articles

The architecture of adenoviruses: recent views and problems: Brief review.

Nermut M Arch Virol. 1980; 64(3):175-96.

PMID: 6996642 DOI: 10.1007/BF01322699.

Virological applications of the grid-cell-culture technique.

Hyatt A, Eaton B Electron Microsc Rev. 1990; 3(1):1-27.

PMID: 1966471 PMC: 7172822. DOI: 10.1016/0892-0354(90)90011-g.

Structural elements in adenovirus cores. Evidence for a "core shell" and linear structures in "relaxed" cores.

Nermut M Arch Virol. 1979; 62(2):101-16.

PMID: 44463 DOI: 10.1007/BF01318063.

References

Nermut M . Fine structure of adenovirus type 5. I. Virus capsid. Virology. 1975; 65(2):480-95. DOI: 10.1016/0042-6822(75)90053-7. View

Tjia S, Fanning E, Schick J, DOERFLER W . Incomplete particles of adenovirus type 2. III. Viral and cellular DNA sequences in incomplete particles. Virology. 1977; 76(1):365-79. DOI: 10.1016/0042-6822(77)90309-9. View

Morgan C, Rosenkranz H, Mednis B . Structure and development of viruses as observed in the electron microscope. V. Entry and uncoating of adenovirus. J Virol. 1969; 4(5):777-96. PMC: 375937. DOI: 10.1128/JVI.4.5.777-796.1969. View

Nermut M, Williams L . Freeze-fracturing of monolayers (capillary layers) of cell, membranes and viruses: some technical considerations. J Microsc. 1977; 110(2):121-32. DOI: 10.1111/j.1365-2818.1977.tb00023.x. View

Rosenwirth B, Tjia S, Westphal M, DOERFLER W . Incomplete particles of adenovirus. II. Kinetics of formation and polypeptide composition of adenovirus type 2. Virology. 1974; 60(2):431-7. DOI: 10.1016/0042-6822(74)90337-7. View

Corden J, Engelking H, Pearson G . Chromatin-like organization of the adenovirus chromosome. Proc Natl Acad Sci U S A. 1976; 73(2):401-4. PMC: 335916. DOI: 10.1073/pnas.73.2.401. View

Everitt E, Lutter L, Philipson L . Structural proteins of adenoviruses. XII. Location and neighbor relationship among proteins of adenovirion type 2 as revealed by enzymatic iodination, immunoprecipitation and chemical cross-linking. Virology. 1975; 67(1):197-208. DOI: 10.1016/0042-6822(75)90417-1. View

Nermut M, HARPST J, Russell W . Electron microscopy of adenovirus cores. J Gen Virol. 1975; 28(1):49-58. DOI: 10.1099/0022-1317-28-1-49. View

Brown D, Westphal M, Burlingham B, Winterhoff U, DOERFLER W . Structure and composition of the adenovirus type 2 core. J Virol. 1975; 16(2):366-87. PMC: 354676. DOI: 10.1128/JVI.16.2.366-387.1975. View

10.

Pereira H, WRIGLEY N . In vitro reconstitution, hexon bonding and handedness of incomplete adenovirus capsid. J Mol Biol. 1974; 85(4):617-30. DOI: 10.1016/0022-2836(74)90319-2. View

11.

Russell W, McIntosh K, Skehel J . The preparation and properties of adenovirus cores. J Gen Virol. 1971; 11(1):35-46. DOI: 10.1099/0022-1317-11-1-35. View

12.

Reynolds E . The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol. 1963; 17:208-12. PMC: 2106263. DOI: 10.1083/jcb.17.1.208. View

13.

Sleytr U, Umrath W . A simple fracturing device for obtaining complementary replicas of freeze-fractured and freeze-etched suspensions and tissue fragments. J Microsc. 1974; 101(Pt 2):177-86. DOI: 10.1111/j.1365-2818.1974.tb03880.x. View

14.

Ishibashi M, Maizel Jr J . The polypeptides of adenovirus. V. Young virions, structural intermediate between top components and aged virions. Virology. 1974; 57(2):409-24. DOI: 10.1016/0042-6822(74)90181-0. View

15.

Franks F, Skaer H . Aqueous glasses as matrices in freeze-fracture electron microscopy. Nature. 1976; 262(5566):323-5. DOI: 10.1038/262323a0. View

16.

Marusyk R, Norrby E, Marusyk H . The relationship of adenovirus-induced paracrystalline structures of the virus core protein(s). J Gen Virol. 1972; 14(3):261-70. DOI: 10.1099/0022-1317-14-3-261. View

17.

Edvardsson B, Everitt E, Jornvall H, Prage L, Philipson L . Intermediates in adenovirus assembly. J Virol. 1976; 19(2):533-47. PMC: 354890. DOI: 10.1128/JVI.19.2.533-547.1976. View

18.

Nermut M, Ward B . Effect of fixatives on fracture plane in red blood cells. J Microsc. 1974; 102(Pt 1):29-39. DOI: 10.1111/j.1365-2818.1974.tb03963.x. View

19.

Fujio K, Ichikawa H, Kumon H, Uno F, Tawara J . Freeze-etching observation on the nucleus of HeLa-S3 cell infected with adenovirus type 12. J Electron Microsc (Tokyo). 1976; 25(4):297-8. View

20.

Chardonnet Y, Dales S . Early events in the interaction of adenoviruses with HeLa cells. II. Comparative observations on the penetration of types 1, 5, 7, and 12. Virology. 1970; 40(3):478-85. DOI: 10.1016/0042-6822(70)90190-x. View