Histone Hyperacetylation Has Little Effect on the Higher Order Folding of Chromatin

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1983 Jun 25

PMID 6866766

Citations 18

Authors

J D McGhee

J M Nickol

G Felsenfeld

D C Rau

Affiliations

Soon will be listed here.

Abstract

HeLa cells were grown in the presence of 10 mM sodium butyrate and soluble chromatin containing hyperacetylated histones was prepared by mild micrococcal nuclease digestion and sucrose gradient fractionation. Sedimentation and electric dichroism were used to study the cation-induced folding of this acetylated chromatin from the 10 nm filament to the 30 nm solenoid conformation. Although under some conditions acetylated chromatin appears slightly less condensed than control chromatin, the major conclusion is that hyperacetylation of histones does not in itself prevent the formation of the higher order chromatin solenoid.

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