Physiological Effects of an Allozyme Polymorphism: Glutamate-pyruvate Transaminase and Response to Hyperosmotic Stress in the Copepod Tigriopus Californicus

Overview

Journal Biochem Genet

Specialty Molecular Biology

Date 1983 Apr 1

PMID 6860293

Citations 18

Authors

R S Burton

M W Feldman

Affiliations

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Abstract

In order to regulate cell volume during hyperosmotic stress, the intertidal copepod Tigriopus californicus, like other aquatic crustaceans, rapidly accumulates high levels of intracellular alanine, proline, and glycine. Glutamate-pyruvate transaminase (GPT; EC 2.6.1.2), which catalyzes the final step of alanine synthesis, is genetically polymorphic in T. californicus populations at Santa Cruz, California. Spectrophotometric studies of homogenates derived from a homozygous isofemale line of each of the two common GPT alleles indicated that the GPTF allozyme has a significantly higher specific activity than the GPTS allozyme. Under conditions of hyperosmotic stress, individual adult copepods of GPTF and GPTF/S genotypes accumulated alanine, but not glycine or proline, more rapidly than GPTS homozygotes. When young larvae were subjected to the same hyperosmotic conditions, GPTS larvae suffered a significantly higher mortality than GPTF or GPTF/S larvae. These results suggest that the biochemical differences among GPT allozymes result in specific physiological variation among GPT genotypes and that this physiological variation is manifested in differential genotypic survivorships under some naturally occurring environmental conditions.

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References

Schopf T, Gooch J . GENE FREQUENCIES IN A MARINE ECTOPROCT: A CLINE IN NATURAL POPULATIONS RELATED TO SEA TEMPERATURE. Evolution. 2017; 25(2):286-289. DOI: 10.1111/j.1558-5646.1971.tb01882.x. View

Watt W . Adaptation at specific loci. I. Natural selection on phosphoglucose isomerase of Colias butterflies: Biochemical and population aspects. Genetics. 1977; 87(1):177-94. PMC: 1213725. DOI: 10.1093/genetics/87.1.177. View

VARGA J, Richards F . Quantitative amino acid compositions at the picomole level using fluorescence scanning. Anal Biochem. 1973; 53(2):397-406. DOI: 10.1016/0003-2697(73)90086-9. View

BULOS B, Handler P . KINETICS OF BEEF HEART GLUTAMIC-ALANINE TRANSAMINASE. J Biol Chem. 1965; 240:3283-94. View

Koehn R . Esterase heterogeneity: dynamics of a polymorphism. Science. 1969; 163(3870):943-4. DOI: 10.1126/science.163.3870.943. View

Place A, Powers D . Genetic variation and relative catalytic efficiencies: lactate dehydrogenase B allozymes of Fundulus heteroclitus. Proc Natl Acad Sci U S A. 1979; 76(5):2354-8. PMC: 383599. DOI: 10.1073/pnas.76.5.2354. View

Clarke B . The contribution of ecological genetics to evolutionary theory: detecting the direct effects of natural selection on particular polymorphic loci. Genetics. 1975; 79 Suppl:101-13. View

Day T, Hillier P, Clarke B . Properties of genetically polymorphic isozymes of alcohol dehydrogenase in Drosophila melanogaster. Biochem Genet. 1974; 11(2):141-53. DOI: 10.1007/BF00485770. View

Schoffeniels E . Adaptations with respect to salinity. Biochem Soc Symp. 1976; (41):179-204. View

10.

Cavener D, Clegg M . Evidence for biochemical and physiological differences between enzyme genotypes in Drosophila melanogaster. Proc Natl Acad Sci U S A. 1981; 78(7):4444-7. PMC: 319807. DOI: 10.1073/pnas.78.7.4444. View

11.

PIPKIN S, Rhodes C, Williams N . Influence of temperature on Drosophila alcohol dehydrogenase polymorphism. J Hered. 1973; 64(4):181-5. DOI: 10.1093/oxfordjournals.jhered.a108384. View

12.

Koehn R, Rasmussen D . Polymorphic and monomorphic serum esterase heterogeneity in catostomid fish populations. Biochem Genet. 1967; 1(2):131-44. DOI: 10.1007/BF00486514. View

13.

Christiansen F, FRYDENBERG O . Geographical patterns of four polymorphisms in Zoarces viviparus as evidence of selection. Genetics. 1974; 77(4):765-70. PMC: 1213166. DOI: 10.1093/genetics/77.4.765. View

14.

Bohlen P, Stein S, Imai K, UDENFRIEND S . A simplified protein assay with fluorescamine in samples containing interfering material. Anal Biochem. 1974; 58(2):559-62. DOI: 10.1016/0003-2697(74)90224-3. View

15.

Burton R, Feldman M . POPULATION GENETICS OF TIGRIOPUS CALIFORNICUS. II. DIFFERENTIATION AMONG NEIGHBORING POPULATIONS. Evolution. 2017; 35(6):1192-1205. DOI: 10.1111/j.1558-5646.1981.tb04989.x. View

16.

Vigue C, Johnson F . Isozyme variability in species of the genus Drosophila. VI. Frequency-property-environment relationships of allelic alcohol dehydrogenases in D. melanogaster. Biochem Genet. 1973; 9(3):213-27. DOI: 10.1007/BF00485735. View

17.

Burton R, Feldman M, Swisher S . Linkage relationships among five enzyme-coding gene loci in the copepod Tigriopus californicus: a genetic confirmation of achiasmiatic meiosis. Biochem Genet. 1981; 19(11-12):1237-45. DOI: 10.1007/BF00484576. View

18.

Doane W . SELECTION FOR AMYLASE ALLOZYMES IN DROSOPHILA MELANOGASTER: SOME QUESTIONS. Evolution. 2017; 34(5):868-874. DOI: 10.1111/j.1558-5646.1980.tb04025.x. View

19.

Nevo E . Genetic variation in natural populations: patterns and theory. Theor Popul Biol. 1978; 13(1):121-77. DOI: 10.1016/0040-5809(78)90039-4. View