Nucleotide Sequence-directed Mapping of the Nucleosomes

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1983 Jun 11

PMID 6856466

Citations 23

Authors

G Mengeritsky

E N Trifonov

Affiliations

Soon will be listed here.

Abstract

The concept of sequence-dependent deformational anisotropy of DNA proposed earlier is further elaborated and a computational procedure is developed for the sequence-directed mapping of the nucleosomes along chromatin DNA nucleotide sequences. The deformational anisotropy is found to be nonuniform along the molecule of the nucleosomal DNA, suggesting that the DNA superhelix in the nucleosome is slightly oval rather than circular in projection. The number of superhelical turns in the nucleosome core particle is estimated to be 2.0 +/- 0.2. Preliminary mapping of the nucleosomes in various chromatin DNA sequences yields the distribution of linker lengths which shows several minima separated by about 10 base-pairs. This is explained by sterical exclusion effects due to overlapping of the nucleosomes in space when some specific linker lengths are chosen. The mapping procedure described is tested by comparing its results with all the most accurate experimental mapping data reported so far. The comparison demonstrates that the exact positions of all the nucleosomes appear to be determined exclusively by the nucleotide sequences.

Citing Articles

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The nucleosome position-encoding WW/SS sequence pattern is depleted in mammalian genes relative to other eukaryotes.

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Preferential nucleosome occupancy at high values of DNA helical rise.

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Variety of genomic DNA patterns for nucleosome positioning.

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