Lipid Deterioration: Beta-carotene Destruction and Oxygen Evolution in a System Containing Lactoperoxidase, Hydrogen Peroxide and Halides

Overview

Journal Lipids

Specialty Biochemistry

Date 1983 Mar 1

PMID 6855479

Citations 3

Authors

J Kanner

J E Kinsella

Affiliations

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Abstract

A model system containing lactoperoxidase/H2O2/halide decomposed beta-carotene in a reaction greatly affected by the concentration of H2O2. The optimal concentrations of H2O2 for activation of iodide and bromide were 2 mM and 10 microM, respectively. The oxidation of chloride by a lactoperoxidase, using beta-carotene destruction as a sensitive method to determine the activity of the enzyme, is reported herein. In the presence of optimal amounts of H2O2, the rate of beta-carotene destruction increases slowly until a critical concentration of the halides, followed by a rapid increase in the rate when halide concentrations were further increased. A lactoperoxidase/H2O2/iodide and/or bromide system generates oxygen in the presence of high H2O2 and halide concentrations. beta-Carotene inhibited the evolution of oxygen. A possible mechanism of beta-carotene destruction and triplet unexcited oxygen evolution by a lactoperoxidase/H2O2/halide system are proposed.

Citing Articles

Initiation of lipid peroxidation by a peroxidase/hydrogen peroxide/halide system.

Kanner J, Kinsella J Lipids. 1983; 18(3):204-10.

PMID: 6855480 DOI: 10.1007/BF02534549.

Carotenoids and retinol: their possible importance in determining longevity of primate species.

Cutler R Proc Natl Acad Sci U S A. 1984; 81(23):7627-31.

PMID: 6594706 PMC: 392201. DOI: 10.1073/pnas.81.23.7627.

Lipid peroxidation and oxidation of several compounds by H2O2 activated metmyoglobin.

Kanner J, Harel S Lipids. 1985; 20(9):625-8.

PMID: 4046748 DOI: 10.1007/BF02534290.

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