Evaluation of the Usefulness of Counterimmunoelectrophoresis for Diagnosis of Clostridium Difficile-associated Colitis in Clinical Specimens

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 1983 Apr 1

PMID 6853688

Citations 10

Authors

T C Wu

J C Fung

Affiliations

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Abstract

Results of counterimmunoelectrophoresis (CIE) were compared with those of isolation of Clostridium difficile and assay for cytotoxicity in HeLa cells. On the basis of 471 stool specimens, CIE exhibited a sensitivity of 38% and a specificity of 88% as compared with the cytotoxin assay. The predictive value of a reactive CIE results is low (17%), whereas the predictive value of a nonreactive CIE result is significant (96%) and therefore warrants its use as a screening test. In addition, stool filtrates may nonspecifically precipitate with the C. difficile antitoxin in the CIE test. Such nonspecific reactions may be identified by simultaneous electrophoresis against nonimmune serum.

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