Altered Rates of Collagen Synthesis in in Vitro Aged Human Lung Fibroblasts

Overview

Journal In Vitro

Specialties Anatomy & Histology
Cell Biology

Date 1983 Apr 1

PMID 6852832

Citations 2

Authors

J N Hildebran

M Absher

R B Low

Affiliations

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Abstract

Absolute rates of protein and collagen synthesis based on prolyl-tRNA as the precursor were determined in two age groups of IMR-90 human lung fibroblasts. Compared with midrange fibroblasts [population doubling level (PDL) = 20 to 30] aged fibroblasts (PDL greater than 40] were larger in size in terms of protein and RNA per cell, generally proliferated more slowly, exhibited different steady state [3H] proline transfer RNA (tRNA) precursor pool specific radioactivities, synthesized collagen at a substantially lower rate, and exhibited a reduction in the percent commitment to collagen synthesis. Total protein synthetic rates were reduced slightly in aged versus midrange fibroblasts but the difference was not statistically significant. Proliferative capacity (PDL/wk) correlated better with these changes than cumulative PDL. Cell size (protein/cell) was the variable that had the highest correlation with the reduction in collagen synthesis observed in human lung fibroblasts. Thus, an important differentiated function of human lung fibroblasts, collagen synthesis, is greatly diminished in vitro in large, slowly dividing fibroblasts.

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References

Airhart J, Vidrich A, Khairallah E . Compartmentation of free amino acids for protein synthesis in rat liver. Biochem J. 1974; 140(3):539-45. PMC: 1168033. DOI: 10.1042/bj1400539. View

Berg R, Schwartz M, Crystal R . Regulation of the production of secretory proteins: intracellular degradation of newly synthesized "defective" collagen. Proc Natl Acad Sci U S A. 1980; 77(8):4746-50. PMC: 349923. DOI: 10.1073/pnas.77.8.4746. View

Peterkofsky B, Diegelmann R . Use of a mixture of proteinase-free collagenases for the specific assay of radioactive collagen in the presence of other proteins. Biochemistry. 1971; 10(6):988-94. DOI: 10.1021/bi00782a009. View

Airhart J, Kelley J, Brayden J, Low R, Stirewalt W . An ultramicro method of amino acid analysis: application to studies of protein metabolism in cultured cells. Anal Biochem. 1979; 96(1):45-55. DOI: 10.1016/0003-2697(79)90552-9. View

Houghton B, STIDWORTHY G . A growth history comparison of the human diploid cells WI-38 and IMR-90: proliferative capacity and cell sizing analysis. In Vitro. 1979; 15(9):697-702. DOI: 10.1007/BF02618249. View

Rannels D, Low R, YOUDALE T, VOLKIN E, LONGMORE W . Use of radioisotopes in quantitative studies of lung metabolism. Fed Proc. 1982; 41(12):2833-9. View

Regier J, Kafatos F . Absolute rates of protein synthesis in sea urchins with specific activity measurements of radioactive leucine and leucyl-tRNA. Dev Biol. 1977; 57(2):270-83. DOI: 10.1016/0012-1606(77)90214-7. View

van Venrooij W, Moonen H, van Loon-Klaassen L . Source of amino acids used for protein synthesis in HeLa cells. Eur J Biochem. 1974; 50(1):297-304. DOI: 10.1111/j.1432-1033.1974.tb03898.x. View

Houck J, Sharma V, Hayflick L . Functional failures of cultured human diploid fibroblasts after continued population doublings. Proc Soc Exp Biol Med. 1971; 137(1):331-3. DOI: 10.3181/00379727-137-35571. View

10.

Tolstoshev P, Haber R, Trapnell B, Crystal R . Procollagen messenger RNA levels and activity and collagen synthesis during the fetal development of sheep lung, tendon, and skin. J Biol Chem. 1981; 256(18):9672-9. View

11.

Hayflick L . The cell biology of aging. J Invest Dermatol. 1979; 73(1):8-14. DOI: 10.1111/1523-1747.ep12532752. View

12.

WATKINS C, Rannels D . Measurement of protein synthesis in rat lungs perfused in situ. Biochem J. 1980; 188(1):269-78. PMC: 1162565. DOI: 10.1042/bj1880269. View

13.

McCoy B, Galdun J, Cohen I . Effects of density and cellular aging on collagen synthesis and growth kinetics in keloid and normal skin fibroblasts. In Vitro. 1982; 18(1):79-86. DOI: 10.1007/BF02796388. View

14.

Bienkowski R, Cowan M, McDonald J, Crystal R . Degradation of newly synthesized collagen. J Biol Chem. 1978; 253(12):4356-63. View

15.

Breul S, Bradley K, HANCE A, Schafer M, Berg R, Crystal R . Control of collagen production by human diploid lung fibroblasts. J Biol Chem. 1980; 255(11):5250-60. View

16.

Pitha J, Stork E, Wimmer E . Protein synthesis during aging of human cells in culture. Direction by polio virus. Exp Cell Res. 1975; 94(2):310-4. DOI: 10.1016/0014-4827(75)90497-8. View

17.

Harley C, Pollard J, Chamberlain J, Stanners C, Goldstein S . Protein synthetic errors do not increase during aging of cultured human fibroblasts. Proc Natl Acad Sci U S A. 1980; 77(4):1885-9. PMC: 348613. DOI: 10.1073/pnas.77.4.1885. View

18.

Fleck A, MUNRO H . The precision of ultraviolet absorption measurements in the Schmidt-Thannhauser procedure for nucleic acid estimation. Biochim Biophys Acta. 1962; 55:571-83. DOI: 10.1016/0006-3002(62)90836-3. View

19.

Macieira-Coelho A, Taboury F . A re-evaluation of the changes in proliferation in human fibroblasts during ageing in vitro. Cell Tissue Kinet. 1982; 15(2):213-24. DOI: 10.1111/j.1365-2184.1982.tb01039.x. View

20.

Holland J, KOHNE D, Doyle M . Analysis of virus replication in ageing human fibroblast cultures. Nature. 1973; 245(5424):316-9. DOI: 10.1038/245316a0. View