Determination of Malonaldehyde in Biological Materials by High-pressure Liquid Chromatography

Overview

Journal Anal Biochem

Publisher Elsevier

Specialty Biochemistry

Date 1983 Jan 1

PMID 6846796

Citations 22

Authors

R P Bird

S S Hung

M Hadley

H H Draper

Affiliations

Soon will be listed here.

Abstract

An HPLC method is described for the determination of malonaldehyde in biological materials. The procedure involves extracting the sample with trichloracetic acid, heating the extract with thiobarbituric acid, separating the thiobarbituric acid-malonaldehyde complex on a mu Bondapak C18 column, and measuring the absorbance using a 546-nm interference filter. The method was found to be specific for malonaldehyde in several food and feed samples. Under routine assay conditions, a coefficient of variability of 7.0% was obtained for samples containing 1-2 microgram of malonaldehyde per gram (instrument detection limit 1 ng). This procedure yields lower values for the concentration of malonaldehyde in food samples than the conventional spectrophotometric procedure based on absorbance of the thiobarbituric acid-malonaldehyde complex at 532 nm.

Citing Articles

GC-MS and GC-MS/MS measurement of malondialdehyde (MDA) in clinical studies: Pre-analytical and clinical considerations.

Tsikas D J Mass Spectrom Adv Clin Lab. 2023; 30:10-24.

PMID: 37637438 PMC: 10458701. DOI: 10.1016/j.jmsacl.2023.08.001.

Exploration of the Potential Mechanism of Faba Bean-Wheat Intercropping to Control Faba Bean Fusarium Wilt Due to Allelopathic Plant Extracts.

Guo Y, Lv J, Dong Y, Dong K ACS Omega. 2021; 6(24):15590-15600.

PMID: 34179603 PMC: 8223211. DOI: 10.1021/acsomega.0c06120.

Cinnamic Acid Increased the Incidence of Wilt by Increasing the Pathogenicity of and Reducing the Physiological and Biochemical Resistance of Faba Bean, Which Was Alleviated by Intercropping With Wheat.

Guo Y, Lv J, Zhao Q, Dong Y, Dong K Front Plant Sci. 2020; 11:608389.

PMID: 33381139 PMC: 7767866. DOI: 10.3389/fpls.2020.608389.

Mammographic density, blood telomere length and lipid peroxidation.

Erdmann N, Harrington L, Martin L Sci Rep. 2017; 7(1):5803.

PMID: 28725051 PMC: 5517610. DOI: 10.1038/s41598-017-06036-y.

Thiobarbituric acid-reactive substances from peroxidized lipids.

Kosugi H, Kojima T, Kikugawa K Lipids. 2016; 24(10):873-81.

PMID: 27520441 DOI: 10.1007/BF02535762.