51Cr Release Cytotoxicity Radioimmunoassay to Detect Immune Cytotoxic Reactions to Rat Schwann Cells in Vitro

Overview

Journal Neurosci Lett

Specialty Neurology

Date 1983 Mar 14

PMID 6843908

Citations 3

Authors

R P Lisak

D Kuchmy

J Pollard

Affiliations

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Abstract

Monolayers of rat Schwann cells incubated with 51Cr were used as targets for a cytotoxicity assay employing rabbit antiserum to galactocerebroside (R anti-GalC). Specific 51Cr release was demonstrated which was heat sensitive and complement-dependent. The assay was readily performed, reproducible and quantitative, and should prove useful in assessing anti-Schwann cell cytotoxic activity of experimental and human serum.

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